The Only Blood Culture for Diagnosis of Bacteremia - Comparative Study of Practice

Current recommendations for the diagnosis of bacteremia based on the embodiment February-March blood cultures separated by a minimum interval of 30 minutes. Each blood culture comprises seeding a pair of aerobic and anaerobic vials inoculated each with 5 to 10 ml of blood. The sensitivity and specificity of this technique depends essentially on the amount of blood removed since there is a direct relationship between the volume of blood inoculated into each flask and the efficiency of the technique. A preliminary study conducted at the University Hospital of Caen found that 14-30% of patients depending on the services had received only one blood culture. In addition, at least four blood cultures in 24 hours were taken for 10 to 20% of patients. The practice of a single blood culture reduces the sensitivity of the analysis due to insufficient total amount of blood collected. The practice of too many blood cultures increases the risk of false positive (presence of contaminating bacteria), generates extra work for healthcare personnel (and laboratory) and represents a significant cost for an unproven benefit.

The investigators propose to evaluate a single blood culture sampling technique with seeding 4 vials (2 aerobic and anaerobic 2).

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Key information

Conditions

Bloodstream Infection Bacteremia Bacterial Infections Bacterial Infections and Mycoses Infections Inflammation Pathologic Processes Pathological Conditions, Signs and Symptoms Sepsis Systemic Inflammatory Response Syndrome

Age range

18 year and older

Sex eligibility

All sexes

Study type

Interventional

Phase

Not applicable

About this study

Blood cultures will be collected from patients admitted with on of the following signs: fever (≥38.5°C), hypothermia (≤36°C), chills or shock. For the first blood culture, 40 mL of blood will be obtained aseptically by a single phlebotomy and equally distributed into two BacT/Alert FA aerobic bottles and two BacT/Alert FN anaerobic bottles (bioMérieux, La-Balme-les-Grottes, France). The four bottles will be labelled from one to four in the following order: aerobic-anaerobic-aerobic-anaerobic. Within the next 24 h, one to three other 20-mL blood cultures consisting of a single pair of aerobic and anaerobic bottles will have to be performed, spaced by a minimum of 30 minutes. Bottles will be incubated for 5 days or until positivity reported by the BacT/Alert 3D instrument.

Who can participate

Healthy volunteers accepted: No

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

Patient who is at least 18 years
Patients admitted to an emergency department of a three CHU Caen, Lille, Rouen
Patient with clinical justifying the realization of blood cultures (as good practice recommendations)
Patients who received information about the study or if his legal representatives or his close entourage
Patient not objecting to the venipuncture

Exclusion criteria

Patient who direct venipuncture is impossible
Patient refusing venipuncture
Patient aged under 18
Patient with impaired vigilance and not accompanied by a family member or a trusted person may receive clear information protocol
Vulnerable Patient and not accompanied by a family member or a trusted person may receive clear information protocol

Treatment and study plan

Single-sampling strategy vs multi-sampling strategy for the diagnosis of bacteremia

Procedure

Comparison between two strategies of blood culture (single-sampling strategy and multi-sampling strategy) for the diagnosis of bacteremia

Primary outcomes

Rate of pathogens identified by each strategy

Time frame: baseline

Secondary outcomes

Proportion of blood cultures contaminated in each strategy

Time frame: baseline

Sponsors and collaborators

Lead sponsor

University Hospital, Caen

Other

Registry information

Acronym: HEMU

Important dates

Study start: 2010
Primary completion: 2012
Study completion: 2012
First posted: Aug 6, 2015
Registry last updated: Aug 6, 2015

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

Published trials that share one or more normalized conditions with this study.

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