The Influence of Liraglutide on the Reward Properties of Food: an fMRI Study on Healthy Volunteers

Design: The study is designed as a double blind, placebo controlled, randomized crossover intervention study with two arms (liraglutide first followed by placebo vs. placebo first followed by liraglutide) in 16 healthy male volunteers. fMRI results and food intake are compared between groups baseline vs. end of treatment period and placebo vs. liraglutide.

Intervention: A standard dose of 0.6 mg Liraglutide (oder placebo) will be used, and will be applied subcutaneously each morning for three subsequent days by the study personnel. Study sessions will be performed on the fourth day after placebo/liraglutide interventions.

Study sessions: On study days participants arrive at the trial centre at 8.00 am after a 12h fast. Participants are instructed to eat a light dinner around 8.00 pm the previous day and to spend around 8h resting during the night before the study days. Upon arrival, blood pressure and heart rate is measured and an iv line is started, from which a blood sample is taken from the antecubital vein for blood glucose, insulin and plasma hormone concentrations determination. The occurrence of nausea, vomiting, dizziness or any other adverse events (AE's) is reviewed together with the subject.

Instructions for the fMRI examination are reviewed together with the subject. The subject is advised to leave all ferromagnetic items behind, and baseline hunger/satiety visual analogue scales (VAS) are recorded. Then, the fMRI session with the food items paradigm (together with repeated recordings of hunger/satiety VAS) takes place. Afterwards, another blood sample and hunger/satiety VAS is taken and the subject is allowed to eat ad libitum from a buffet breakfast. Food intake is recorded in detail, including meal choices and calorie intake. After a final hunger/satiety VAS recording and another blood sample subjects are dismissed from the trial centre.

Outcome variables: Main outcome variables are fMRI activity in the brain during the high calorie/low calorie food items paradigm and food intake at the buffet liraglutide vs. placebo at the end of treatment. Secondary outcome parameters are hunger/satiety VAS ratings, total and active plasma ghrelin, peptide YY (PYY), glucose and insulin concentrations.

Inclusion criteria

• Male healthy volunteers
• Age 20-40
• Non-smokers
• Normal body weight (body mass index 19 -25 kg/m2)
• Right handed
• Signed informed consent
• Willingness and ability to comply with the protocol

Exclusion criteria

• Any history of or current drug abuse including alcohol consumption on a regular basis or binge drinking
• Any history of or current psychiatric disease, including any eating disorder, as assessed by structured interview
• Any history of dieting
• Any condition interfering with fMRI measurements such as ferromagnetic implants or claustrophobia
• Any evidence of relevant renal, liver, thyroid, cardiovascular, or respiratory illness at screening examination
• Any acute illness necessitating medical treatment during the last 3 weeks before study entry, any permanent intake of medication
• Any study participation in the last 3 months
• HIV, hepatitis B or C positive
• Any disease considered relevant for proper performance of the study or risks to the participant, at the discretion of the investigator