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NCT Number: NCT06703554

The Impact of Sage Extract on GTS B and D Gene Expression in S. Mutans

Glucosyltransferases (Gtfs) play a crucial role in the pathogenesis of dental caries caused by Streptococcus mutans. Gtfs is recognized as an attractive target for effective therapeutic interventions aimed at inhibiting cariogenic biofilms.

Objective: This study evaluates the antibacterial effects of sage extract compared to chlorhexidine mouthwashes on the expression of Gtf genes in S. mutans from salivary samples of high caries risk patients over a one-month follow-up, utilizing real-time polymerase chain reaction.

Methodology: This clinical trial involves 42 participants with a high risk for caries, who are randomly divided into two groups of 21 subjects each.

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Key information

Conditions

Caries,Dental Dental Caries Dental Diseases Stomatognathic Diseases Tooth Demineralization Tooth Diseases

Age range

20 year–50 year

Sex eligibility

All sexes

Study type

Interventional

Phase

Not applicable

Primary location

Cairo University

Cairo, Egypt

About this study

Glucosyltransferases (Gtfs) are essential in the development of dental caries caused by Streptococcus mutans. They are currently recognized as promising targets for effective therapeutic strategies aimed at disrupting cariogenic biofilms.

Objective: This study investigates the antibacterial effects of sage extract in comparison to chlorhexidine mouthwashes on the expression of Gtf genes in S. mutans from the saliva of patients at high risk for caries, over a one-month follow-up period, using real-time polymerase chain reaction.

Methodology: This clinical trial includes 42 high caries risk participants, who are randomly assigned to two groups, each consisting of 21 subjects.

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • medically free, high caries patient with DMFT

Exclusion criteria

  • pregnant and smokers

Treatment and study plan

Sage Oil Mouthwash

Other

Sage oil obtained from a local market of herbal products in Egypt. In order to identify chemical composition of sage essential oils and overall purity, Gas Chromatography/ Mass Spectrometry analysis (GC/MS) performed

Other names: Natural product

Chlorohexidine Mouthwash

Biological

0.12% chlorhexidine gluconate in a base containing water, 11.6% alcohol, glycerin, PEG-40 sorbitan diisostearate, flavor, and sodium saccharin

Other names: CHX mouthwash

Primary outcomes

  1. real-time PCR

    Time frame: 1 week

    S. Mutans, Gtf B, Gtf D

  2. real-time PCR

    Time frame: 2 weeks

    S. Mutans, Gtf B, Gtf D

  3. real-time PCR

    Time frame: 3 weeks

    S. Mutans, Gtf B, Gtf D

  4. real-time PCR

    Time frame: 4 weeks

    S. Mutans, Gtf B, Gtf D

  5. Gene Expression

    Time frame: 1 week

    S. Mutans, Gene Expression

  6. Gene Expression

    Time frame: 2 weeks

    S. Mutans, Gene Expression

  7. Gene Expression

    Time frame: 3 weeks

    S. Mutans, Gene Expression

  8. Gene Expression

    Time frame: 4 weeks

    S. Mutans, Gene Expression

Sponsors and collaborators

Lead sponsor

Cairo University

Other

Registry information

Official study title

The Impact of Natural Sage Extract on Glucosyltransferase B and D Gene Expression in Streptococcus Mutans: A Randomized Clinical Trial Using Real Time Polymerase Chain Reaction

Important dates

Study start
2024
Primary completion
2024
Study completion
2024
First posted
Nov 25, 2024
Registry last updated
May 7, 2025

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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