The Impact of Low Level Laser Treatment on Skeletal Muscle and Skin Tissue

The present study utilizes an acute within-subject design in healthy young adult participants. In total, 12 healthy young adults (6 men and 6 women) will participate in the study. Participants' legs will be randomly assigned to low level laser treatment or no treatment (Figure 1). Each participant will participate in a screening session (~1 h) and 1 experimental test day (~1.5 h). Figure 1 shows a graphical illustration of the study overview.

In total, 12 healthy (BMI 18.5-30 kg/m2) young (age: 18-35 y) males and females will participate in the present study. The nature and the risks of the experimental procedures will be explained to all subjects before their informed consent will be obtained. All subjects will be recruited through social media and advertisements on dedicated bulletin boards within the MUMC+ buildings and local shops will be used.

Each subject will participate in an experimental trial lasting ~1.5 h. Subjects will be instructed to arrive at the university at 9:00 AM in an overnight fasted and rested state, meaning that participants are not allowed to eat and drink (except for water) from 21:00 the night prior to the experimental trial. They will be instructed to come to the university by car or public transportation. After the subjects arrive at the University, we will ask them to put on their shorts, determine their body mass, and assign them to a bed. Subjects will rest in a supine position for 10 minutes. Thereafter, the LLLT will be applied on one leg, which will be randomized. The other leg will receive no treatment. Immediately after the treatment muscle biopsies will be taken from the m. vastus lateralis of both legs. The muscle biopsy from the treated leg will be sampled 15 min after finishing the LLLT. Additionally, skin biopsies will be taken from the same area. The skin biopsy of the treated leg will be collected 20 min after finishing the LTT. During the test day, a total of 2 muscle and skin biopsies will be taken, 1 biopsy from each leg. Here each skin biopsy sampling consists of 2 4 mm punch biopsies. The time between finishing the LLLT and collection of the tissue samples will be noted.

Primary objective: To assess the impact of acute laser treatment on muscle tissue mitochondrial respiration in vivo in healthy, young adults.

Secondary objective: To assess the impact of acute laser treatment on muscle cellular energy, anabolic, angiogenic and inflammatory pathways, along with enzyme activity.

Tertiary objectives: To assess the impact of acute laser treatment on skin cellular energy, anabolic, angiogenic and inflammatory pathways, along with enzyme activity.

Hypothesis:

• It is hypothesized that muscle mitochondrial respiration will be higher following low level laser treatment.
• It is hypothesized that muscle gene expression, protein signalling and enzyme activity will be higher following low level laser treatment.
• It is hypothesized that skin gene expression, protein signalling and enzyme activity will be higher following low level laser treatment.

Inclusion criteria

• Male or female sex
• Aged between 18 and 35 y inclusive
• BMI between 18.5 and 30 kg/m2

Exclusion criteria

• Participating in a structured (progressive) exercise program or >4h of vigorous physical activity per week.
• Smoking regularly (i.e. >5 cigarettes/week)
• Pregnancy
• Hormonal replacement therapy
• Diagnosed musculoskeletal disorders
• Diagnosed metabolic disorders (e.g. diabetes)
• Diagnosed skin disorders
• Use of any medications known to affect protein metabolism (i.e. corticosteroids, non-steroidal anti-inflammatories).
• Chronic use of anti-coagulants