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Completed

NCT Number: NCT06264193

The Impact of Hypoxia on the Biochemical and Morphological Parameters of Blood in Rowers.

The response to hypoxia is very individual and epending on many aspects, such as the type of training, duration, intensity, or hypoxic stimulus, hypoxia affects the athlete in various ways. The results of this study have shown that 18 days of the LH-TL method does not significantly increase the level of EPO and VEGF in rowers. However, reticulocytes, immature red blood cells, have shown significant differences after 18-d LH-TL between groups. Further research should be carried out to investigate an optimal hypoxic dose and time, which will raise EPO, VEGF, and morphology variables.

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Key information

Sex eligibility

Male

Study type

Interventional

Phase

Not applicable

About this study

The Polish national rower team athletes, 13 males (ages 27,0±4,2 years), were randomly divided into two groups: 1) The hypoxic group (H) included 8 rowers participating in sports training and the LH-TL method, hypoxic rooms (FiO2=14.5%, corresponds to an altitude of 3000 meters). 2) The control group (C) presented 5 rowers participating only in sports training and living in normoxic rooms, at the same sports camp. Rowers were observed during the preparatory period. The hypoxic group lived and slept in hypoxic rooms, for at least 8-14 hours per day. During the camp, all rowers followed the same training schedule and diet.All participants' saturation and heart rate were measured daily, morning and evening, throughout the camp period. Venous blood samples were taken four times, at the beginning of the camp (Baseline), after 5 days, after 12 days, and after 18 days of the camp. Body composition parameters such as body mass index, body fat, and free fat mass were estimated using the bioelectrical impedance method of an analyzer Tanita BC 418 (Japan). Data analysis was performed using Statistica. The response to hypoxia is very individual. Depending on many aspects, such as the type of training, duration, intensity, or hypoxic stimulus, hypoxia affects the athlete in various ways. The results of this study have shown that the blood parameters did not increase significantly in the hypoxic group compared to the control group. However, reticulocytes, immature red blood cells, have shown significant differences after 18-d LH-TL between groups. Further research should be carried out to investigate an optimal hypoxic dose and time, which will raise EPO, VEGF, and morphology variables.

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • member of national rower's teams, and sports camp participant.

Exclusion criteria

  • negative symptoms of hypoxia, and/or injury.

Treatment and study plan

Hypoxia

Other

The athletes were living in hypoxic rooms, and training was carried out on lowlands.

Primary outcomes

  1. Erythropoietin (EPO) mlU/ml

    Time frame: At the beginning of the camp (Baseline), after 5 days (T1), after 12 days (T2), and after 18 days (T3) of the camp.

    immunoenzymatic assay methods using diagnostic kits

  2. vascular endothelial growth factor (VEGF) mlU/ml

    Time frame: At the beginning of the camp (Baseline), after 5 days (T1), after 12 days (T2), and after 18 days (T3) of the camp.

    immunoenzymatic assay methods using diagnostic kits

Secondary outcomes

  1. creatine kinase (CK) ng/ml

    Time frame: At the beginning of the camp (Baseline), after 5 days (T1), after 12 days (T2), and after 18 days (T3) of the camp.

    was determined using spectrophotometer analysis and diagnostic kits

  2. Hs C-Reactive Protein (hsCRP) mg/L

    Time frame: At the beginning of the camp (Baseline), after 5 days (T1), after 12 days (T2), and after 18 days (T3) of the camp.

    was determined by immunoenzymatic assay methods using diagnostic kits

  3. hemoglobin (Hb)g/dL

    Time frame: At the beginning of the camp (Baseline), after 5 days (T1), after 12 days (T2), and after 18 days (T3) of the camp.

    A professional accredited laboratory company determined the Hb level (Poland, ISO 9001:2008).

  4. hematocrit (Htc)%

    Time frame: At the beginning of the camp (Baseline), after 5 days (T1), after 12 days (T2), and after 18 days (T3) of the camp.

    A professional accredited laboratory company determined the Htc level (Poland, ISO 9001:2008).

  5. red blood cells (RBC)mln/mm3

    Time frame: At the beginning of the camp (Baseline), after 5 days (T1), after 12 days (T2), and after 18 days (T3) of the camp.

    A professional accredited laboratory company determined the RBC level (Poland, ISO 9001:2008).

  6. white blood cells (WBC)10 3/µL

    Time frame: At the beginning of the camp (Baseline), after 5 days (T1), after 12 days (T2), and after 18 days (T3) of the camp.

    A professional accredited laboratory company determined the WBC level (Poland, ISO 9001:2008).

  7. reticulocytes (Ret)‰

    Time frame: At the beginning of the camp (Baseline), after 5 days (T1), after 12 days (T2), and after 18 days (T3) of the camp.

    A professional accredited laboratory company determined the Ret level (Poland, ISO 9001:2008).

  8. SpO2 - oxygen saturation (%)

    Time frame: every day, morning and evening, while sports camp

    was measured using a pulse oximeter (Tech-Med, ISO: 13485).

  9. bpm - beats per minute(%)

    Time frame: every day, morning and evening, while sports camp

    was measured using a pulse oximeter (Tech-Med, ISO: 13485).

Sponsors and collaborators

Lead sponsor

Poznan University of Physical Education

Other

Registry information

Official study title

The Effects of Live High-train Low on EPO, VEGF, and Hematological Variables in Elite Rowers

Acronym: IHOBMPBR

Important dates

Study start
2014
Primary completion
2014
Study completion
2014
First posted
Feb 16, 2024
Registry last updated
Jan 17, 2025

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

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This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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