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Completed

NCT Number: NCT06855979

The Impact of Dietary Intervention on Oxidative/antioxidant Markers and Gut Microbiota in Athletes

During intense physical exercise, there is an overproduction of reactive oxygen species, which leads to oxidative stress and reduced training and sports performance, as well as the development of chronic diseases. Eating foods with a high content of bioactive ingredients and high antioxidant potential can alleviate the negative effects caused by reactive oxygen species and improve the state of intestinal microflora.

The aim of these interventional studies was to determine whether daily consumption of foods with high antioxidant potential, including fruit and nut bars, for a period of 1 month would reduce oxidative stress in athletes during competition and positively change the intestinal microflora.

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Key information

Conditions

Non Communicable Diseases Cardiovascular Diseases Disease Attributes Noncommunicable Diseases Pathologic Processes Pathological Conditions, Signs and Symptoms

Age range

20 year–50 year

Sex eligibility

All sexes

Study type

Interventional

Phase

Not applicable

Primary location

Medical University of Bialystok

Bialystok, 15-089, Poland

About this study

The study concerns a 4-week dietary intervention. The intervention consisted of daily consumption of 1 bar weighing 50 g, containing nuts and dried fruits with high antioxidant potential. The study included a group of 50 women and men, healthy athletes (cyclists, long-distance runners and triathletes). A 3-day, 24-hour dietary interview was conducted before and after the dietary intervention. Then, using the Dieta 6.0 computer program, the energy and nutrient contents in the diet were estimated. In addition, adherence to the dietary intervention was assessed using a survey questionnaire. Before and after the dietary intervention, oxidative-antioxidant markers were determined in the blood of the subjects (oxidative stress, antioxidant potential, concentration of antioxidant enzymes: glutathione peroxidase, superoxide dismutase, catalase) using ready-made kits. Moreover, changes in the intestinal microbiome were assessed in feces before and after the dietary intervention. Additionally, height and weight were measured before and after the intervention to calculate body mass index (BMI). The study will help answer the question of whether dietary intervention using foods with high antioxidant potential can influence changes in oxidative-antioxidant markers in the blood, intestinal microbiome and body weight of study participants.

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • healthy men and women with high physical activity (triathletes, cyclists, long-distance runners), not taking medications for chronic diseases, age 20-50 years, men and women.

Exclusion criteria

  • occurence of chronic diseases (e.g. hypertension, diabetes, cancer, thyroid diseases, neurodegenerative diseases), taking medications for chronic diseases, age<20 and >50 years, low physical activity

Treatment and study plan

Food with high antioxidant potential

Other

The dietary intervention using fruit and nut bars with high antioxidant activity will last 1 month. Before and after the procedure, oxidative-antioxidant markers in the blood and intestinal microflora in the stool will be determined.

Primary outcomes

  1. Change in antioxidant potential after dietary intervention

    Time frame: 4 weeks

    Before and after the dietary intervention, the antioxidant potential in the blood was determined using the ABTS method.

  2. Change in gut microbiota after dietary intervention

    Time frame: 4 weeks

    The gut microbiota in feces was determined before and after the dietary intervention in a hospital laboratory.

  3. Assessment of dietary intervention

    Time frame: 4 weeks

    The dietary intervention lasted 4 weeks. The subjects received one bar a day weighing 50 g, containing nuts and dried fruits with high antioxidant potential and high polyphenol content. After the dietary intervention, the participants completed a survey containing questions about compliance with the recommendations of the dietary intervention.

  4. Change in blood glutathione peroxidase concentration after dietary intervention

    Time frame: 4 weeks

    Before and after the dietary intervention, the concentration of glutathione peroxidase enzyme in blood was assessed using ready-made laboratory kits.

  5. Change in superoxide dismutase concentration after dietary intervention

    Time frame: 4 weeks

    Before and after the dietary intervention, the concentration of superoxide dismutase enzyme in blood was assessed using ready-made laboratory kits.

  6. Change in catalase concentrations after dietary intervention

    Time frame: 4 weeks

    Before and after the dietary intervention, the concentration of catalase enzyme in blood was assessed using ready-made laboratory kits.

  7. Change in oxidative stress after dietary intervention

    Time frame: 4 weeks

    Before and after the dietary intervention, the oxidative stress in blood was assessed using ready-made laboratory kits.

Secondary outcomes

  1. Assessment of dietary nutrients before and after dietary intervention

    Time frame: 4 weeks

    Before and after the dietary intervention, a 3-day, 24-hour interview was conducted regarding the consumption of products and dishes during the day. Using the Diet 6.0 computer program, the content of individual nutrients in the diet and energy of diet were determined.

  2. Body weight assessment before and after dietary intervention

    Time frame: 4 weeks

    The change in body weight of the subjects before and after the dietary intervention was determined using a scale in kilograms with an accuracy of 100 g.

  3. Measuring the height of study participants

    Time frame: 4 weeks

    Height was determined in centimeters using a measuring tape with an accuracy of 1 cm.

Sponsors and collaborators

Lead sponsor

Medical University of Bialystok

Other

Registry information

Official study title

The Impact of Dietary Intervention Using Innovative Food with High Antioxidant Potential on Oxidative/antioxidant Markers in Blood and Gut Microbiota in Athletes

Acronym: SportDiet

Important dates

Study start
2025
Primary completion
2025
Study completion
2025
First posted
Mar 4, 2025
Registry last updated
Mar 4, 2025

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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