The Efficiency of Transcranial Direct Current Stimulation in the Treatment of Anorexia Nervosa

The severity of symptoms will be assessed with Eating Attitudes Test (EAT-26)

The severity of symptoms will be assessed with Eating Disorder Examination Questionnaire (EDE-Q 6.0).

To evaluate changes in eating habits and opinions about food and nutrition the Questionnaire of Eating Behaviours (QEB) will be used. It is a qualitative questionnaire designed to study eating habits and opinions about food and nutrition. The first part of the questionnaire covers the frequency of consumption of selected products, the frequency of eating meals and their typical composition. In the second part of the questionnaire, there are 26 statements about food and nutrition, which enable the assessment of the level of nutritional knowledge of the respondents.

Food Intake Variety will be assessed with The Variety Food Consumption Questionnaire. It is a qualitative food consumption frequency questionnaire. It is a simple tool to collect information on the consumption of (yes / no) 63 assortment groups of products.

The severity of stress will be assessed with Perceived Stress Scale (PSS-10). This test consists 10 questions and is used to measure stress based on subjective feelings related to problems and personal events, behavior, coping with stress.

Severity of depressive symptoms will be assessed with Beck's Depression Inventory (BDI)

Severity of depressive symptoms will be assessed with Questionnaire for the Diagnosis of Depression in Children and Adolescents (CDI2).

To assess metacognitive abilities Meta-Cognition Questionnaire (MCQ-A) will be used. Reliable and valid instrument for measuring meta-cognitive beliefs in adolescents (12-18 lat). The MCQ-A assesses the severity of dysfunctional meta-cognitive beliefs in adolescents (12-18) that affect their daily functioning. The first and second subscales measures "positive and negative beliefs about worry," Subscale 3 measures "cognitive confidence," Subscale 4 measures "beliefs about superstition, punishment and responsibility". Finally, subscale 5 measures "cognitive self-consciousness,".

To assess levels of attention Color connection test (CTT-1, CTT-2) and Test of attention and perceptiveness will be used.

To assess intensity of intrusive thoughts CY-BOCS scale will be used

To assess intensity of rumination the Ruminance-Reflectivity Questionnaire will be used

To assess visual memory test BENTON will be used. This test is used to test memory, visual perception and graphomotor skills. The examined person draws the presented patterns (10 pieces) from memory or redraws them, depending on the version and methods of the research carried out by the researcher.

To assess body image self-esteem Appearance Self-Rating Sheet (ASRS) will be used. Assessment of 25 dimensions related to your body - (different parts of your body). The patient receives a visualization of the body figure on the drawing and evaluates its individual elements. The test allows you to assess the importance of appearance for patients, the assessment of satisfaction with one's appearance and the satisfaction index. The SES self-assessment questionnaire will also be used

To assess cognitive abilities Matching Familiar Figures Test (MFF)

To assess cognitive abilities Verbal Fluency Test will be used.

The Rosenberg Self-Esteem Scale (SES) will be used. SES is a tool for assessing the level of general self-esteem - a relatively stable disposition understood as a conscious attitude (positive or negative) towards the Self

A shortened 16-item version of a questionnaire designed for the rapid assessment of difficulties in emotion regulation Difficulties in Emotion Regulation Scale DERS-16 wil be performed. The scale measures five domains, such as lack of emotional acceptance, impulsivity, and difficulties with goal-directed behavior, using a 5-point scale.

The Autism Spectrum Quotient (AQ) is a self-administered questionnaire used to measure the degree to which a person shows autistic traits.

The questionnaire Mentalization Scale (MentS) is used for a rapid assessment of the capacity to reflect on mental states (emotions, intentions, and beliefs).

Family Talk Questionnaire Fat Talk refers to negative, body-related conversations between girls or young women.

A neuropsychological diagnostic tool Rey-Osterrieth Complex Figure used to assess visuospatial abilities, visual memory, and executive functions (planning and organization).

The STAI (State-Trait Anxiety Inventory) is a psychological tool used to measure anxiety as both a state (a temporary feeling of tension) and a trait (a stable personality disposition). It consists of two subscales (X-1 and X-2, or Y-1 and Y-2), each containing 20 questions, with scores ranging from 20 to 80 points.

To evaluate electrolyte levels (mmol/l) in blood serum following tests will be performed:

sodium, phosphorus, magnesium, calcium, potasium

To evaluate morphotic elements complete morphology with smear will be performed:

RBC - Red Blood Cell (M/µl)

To evaluate morphotic elements complete morphology with smear will be performed:

Hemoglobin (mmol/l) Mean Corpuscular Hemoglobin Concentration (mmol/l)

To evaluate morphotic elements complete morphology with smear will be performed:

Hematocrit

To evaluate morphotic elements complete morphology with smear will be performed:

Mean Corpuscular Volume (fl)

To evaluate morphotic elements complete morphology with smear will be performed:

Mean Corpuscular Hemoglobin (pg)

To evaluate morphotic elements complete morphology with smear will be performed:

Ferritin ( ng/ml)

To evaluate morphotic elements complete morphology with smear will be performed:

White Blood Cell (K/µl) Lymphocytes (K/µl)

To evaluate morphotic elements complete morphology with smear will be performed:

Monocytes (G/l)

To evaluate metabolic changes in blood serum Thyroid parameters will be examined: FT3 (pmol/l), FT4 (pmol/l)

To evaluate metabolic changes in blood serum the pituitary gland parameter will be examined: TSH (µIU/ml)

To evaluate neurophysiologic changes EEG (theta and alpha wave power) test will be performed

To evaluate neurotrophin levels in blood serum tests will be performed: BDNF (pg/ml), Neurotrphin 3 and 4 (pg/ml), NGF (pg/ml)

To evaluate food intake regulators in blood serum tests will be performed: leptin (pg/ml), visfatin (pg/ml)

To evaluate body composition changes BIA body composition analysis will be performed: Body weight (kg)

To evaluate body composition changes BIA body composition analysis will be performed: Intracellular and extracellular water (l/%)

To evaluate body composition changes BIA body composition analysis will be performed: Lean body mass (kg/%)

To evaluate body composition changes BIA body composition analysis will be performed: Fat mass (kg/%)

To evaluate HPA axis cortisol (nmol/l) in blood serum will be tested

To evaluate metabolic changes in blood serum Total Protein parameter will be examined.

To evaluate metabolic changes in blood serum the glucosis level will be performed.

To evaluate changes in cardiac parameters in blood serum, NT-proBNP will be measured (pg/mL).

To evaluate metabolic changes in blood serum, a lipid profile parameter will be measured: total cholesterol (mg/dL)

To evaluate metabolic changes in blood serum, a lipid profile parameter will be measured: LDL cholesterol (mg/dL).

To evaluate metabolic changes in blood serum, a lipid profile parameter will be measured: triglycerides (mg/dL).

To evaluate metabolic changes in blood serum, a lipid profile parameter will be measured: non-HDL cholesterol (mg/dL).