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Completed

NCT Number: NCT04713436

The Effects of Antibiotic Combinations on Stem Cells

The purpose of this study was to evaluate the indirect effects of antibiotic combinations. SCAP viability, proliferation and apoptosis of the cells were evaluated. Osteocalcin (BGLAP), dentin sialo phosphoprotein (DSPP) and alkaline phosphatase (ALPP) gene expression levels were also evaluated.

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Key information

Conditions

Stem Cells Anti-bacterial Agents

Age range

16 year–22 year

Sex eligibility

All sexes

Study type

Observational

Primary location

Eskişehir Osmangazi University

Eskişehir, 26040, Turkey (Türkiye)

About this study

The purpose of this study was to evaluate the indirect effects of triple (TAP- ciprofloxacin, metronidazole and minocycline), double (DAP- ciprofloxacin and metronidazole) and modified triple antibiotic pastes (mTAP- ciprofloxacin, metronidazole and cefaclor) on mesenchmal stem cells derived from apical papilla (SCAP). SCAP were isolated from the impacted third molar teeth of the young adults. TAP, DAP and mTAP treatment (14-day medication and 28-day medication) were applied on SCAP and viability, proliferation and apoptosis of the cells were evaluated after four weeks. Osteocalcin (BGLAP), dentin sialo phosphoprotein (DSPP) and alkaline phosphatase (ALPP) gene expression levels were also evaluated.

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • Patients with open apex third molar teeth for extraction indication

Exclusion criteria

  • Patients with mature apex third molar teeth for extraction indication

Treatment and study plan

Differentiation Markers

Drug

Osteocalcin (BGLAP), dentin sialo phosphoprotein (DSPP) and alkaline phosphatase (ALPP) gene expression levels were also evaluated.

Other names: Gene expression levels

Primary outcomes

  1. Measurment of Cell Viability and Apoptosis

    Time frame: 1 year

    To evaluate cell viability and apoptosis MuseCell Analyzer Count and Viability kit (Merck Millipore, Taufkirchen, Germany) was used according to the manufacturer's protocol.

  2. Measurment of cell differantiation

    Time frame: 1 year

    To determine gene expression levels, total RNA of SCAP was extracted using the Ambion PureLink RNA mini kit (Invitrogen, US) and the Roche cell RNA Kit (Penzberg, Germany) according to the manufacturer's instructions.

Sponsors and collaborators

Lead sponsor

Eskisehir Osmangazi University

Other

Collaborators

  • TC Erciyes University

Registry information

Official study title

The Effects of Antibiotic Combinations on Mesenchymal Stem Cells Derived From Apical Papilla

Important dates

Study start
2014
Primary completion
2016
Study completion
2017
First posted
Jan 19, 2021
Registry last updated
Jan 19, 2021

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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