The Effect of PPB Using 10, 20 and 30 ml of Lidocaine, Study on Volunteers

A total number of 40 volunteers will be enrolled in the study. Each volunteer will receive two peripheral nerve blocks (one in each leg) on the day of participation. This will result in 80 peripheral nerve blocks. The types of nerve blocks given to each leg on each volunteer will depend on randomization.

The 80 nerve blocks are divided into five groups specified by type of nerve block, volume of anesthetic used, and respective numbers of legs used in the group. The groups and specifications are listed here:

Groups name, type of peripheral nerve block, study medication dosage and number of legs in the group:

• Group A: PPB with 10 ml of lidocaine 1%, 20 legs.
• Group B: PPB with 20 ml of lidocaine 1%, 20 legs.
• Group C: PPB with 30 ml of lidocaine 1%, 20 legs.
• Group FNB: FNB with 20 ml of lidocaine 1%, 10 legs.
• Group SNB: SNB with 20 ml of lidocaine 1%, 10 legs.

Group FNB of SNB are performed unblinded and works as active comparatives for model control, in order to establish reference points for when a nerve is considered affected.

Prior to nerve block procedure , pre block assessments are obtained in the following order:

• Sensory test of the saphenous nerve.
• MVIC by ankle plantarflexion.
• MVIC by ankle dorsiflexion.
• MVIC by knee extension.
• Compound muscle action potential (cMAP) recordings in the following order:

• Nerve to vastus medialis muscle
• Nerve to vastus lateralis muscle
• Nerve to tibialis anterior muscle
• Nerve to gastrocnemius muscle

60 minutes after the block performance, post block assessments are done. Values are obtained in the same order as for the pre block assessments.

A peripheral intravenous line is inserted prior to the procedure of the peripheral nerve block is performed. At the discretion of the anesthetist, up to 20 mg Propofol IV may be provided to ease the patient during the nerve block procedure. The volunteer is monitored with continuous electrocardiography and pulse oximetry for the first 30 minutes after block performance and clinically hereafter. Final check by an investigator to ensure the volunteer is ready to go home. If the muscle strength is reduced, the volunteer will not be sent home before the ability to walk safely with crutches is ensured.

Inclusion criteria

• ASA 1-2
• Ability to give their written informed consent to participating in the study after having fully understood the contents of the study

Exclusion criteria

• Subjects who cannot cooperate with the study.
• Subjects who cannot understand or speak Danish.
• Subjects with allergy to the medicines used in the study.
• Subjects suffering from alcohol and/or drug abuse - based on the investigator's opinion.
• Pathology or previous major surgery to the lower limb.
• Intake of any analgesics 24 hours prior to baseline measurements.
• BMI > 35
• Active signs of infection in the cutaneous area of injection
• Subjects diagnosed with epilepsy, neurologic diseases, severe hypoxia or respiratory depression, hypovolemia, shock, bradycardia or Wolff-Parkinson-Whites syndrome
• Subjects with a positive pregnancy test**
• Subjects diagnosed with cardiovascular disease or heart failure
• Subjects diagnosed with partial or complete cardiac block
• Subjects in daily treatment with class III antiarrhythmics (e.g. amiodarone)
• Subjects in daily treatment with drugs that are structurally related to local analgesics or class IB antiarrhythmics
• Subjects diagnosed with severe liver disease or reduced kidney function