The Effect of Nutrient Intake on the Microbiome, Weight, and Glucoregulation (NI-MWG)

Inclusion criteria

• Female
• Age 18-65 (inclusive, at time of informed consent)
• BMI ≥ 30 kg/m2 with T2DM
• BMI ≥ 30 kg/m2 without T2DM
• Normal weight lean controls without T2DM

Exclusion criteria

• Tobacco use in past three months - will add unnecessary confound to the data.
• Taking a medication on a routine/recent basis which is known to significantly influence gastrointestinal transit time or affect the microbiome or other variables significantly (as determined by study pharmacist/MD)
• Has taken an oral or injectable antibiotic in the past 3 months
• Has taken a commercially prepared probiotic and/or prebiotic agent in the 3 months
• History of significant intestinal disease or disorder (e.g., crohn's disease, ulcerative colitis)
• History of gastrointestinal surgery that may impact measures of biological variables, as determined by the investigator
• Medical condition expected to impact the biological variables of interest or interfere with providing a sample, as determined by the investigator.
• Unable to speak/read English
• Breastfeeding, pregnant, or planning to become pregnant within the duration of the study as assessed through self-report on medical history
• Unwilling to use a medically acceptable form of contraception during study involvement. Medically acceptable forms of contraception include oral contraception, physical barrier methods and/or abstinence.
• Any known infectious disease such as Viral Hepatitis or HIV (as determined by study pharmacist/MD)