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OpenTrials
Completed

NCT Number: NCT01389492

The Effect of Meat and Food Drived Polyphenols on Oxidize LDL Level

The postprandial increase in MDA level following meat meal could cause modification to LDL particles in healthy volunteers. Co-consumption of food drived polyphenols with the meat will result in a significant decrease in LDL modification.

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Key information

Conditions

Healthy

Age range

18 year–60 year

Sex eligibility

All sexes

Study type

Interventional

Phase

Not applicable

Primary location

The Hebrew University of Jerusalem

Jerusalem, 91120, Israel

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • Healthy adults, normal BMI

Exclusion criteria

  • Metabolic disorders
  • Smokers
  • Drinkers
  • Taking nutritional supplement

Treatment and study plan

frozen meat cutlets meal

Other

250 g of meat cutlets

meat meals

Other

250 g of meat meal for 4 days

Primary outcomes

  1. Change in plasma MDA levels following meat meal

    Time frame: Fasting before meal and 3, and 6 h postprandial, and fasting levels

    After an overnight fast baseline (0 h) fasting blood sample (10 ml) was collected in EDTA-treated tubes. The subjects then ate a test meal within 30 min. Blood samples (10 ml) were drawn 3 and 6 h after the meal was eaten. Serum was separated from whole blood by centrifugation (910 g, 15 min), and MDA was evaluated by HPLC in plasma samples

  2. Changes in MDA concentration on LDL fraction following meat meals

    Time frame: Fasting, before meal was eaten, 3 h following meal, at day 4, 3 h following meal consumption, and day 5 following over nigth fast

    Subject consumed the test meals for 4 days. Day 1, (0 h) fasting blood sample (10 ml) was collected in EDTA-treated tubes. The sbjects then ate a test meal within 30 min. Blood samples (10 ml) were drawn 3 h after the meal was eaten. Blood was collected at day 4, 3 h after the meal was eaten, at the morning of day 5.Plasma LDL was isolated using a single discontinuous density gradient ultracentrifugation. The protein concentration was determined based on the method of Bradford. MDA concentration was evaluated by HPLC.

Sponsors and collaborators

Lead sponsor

Hadassah Medical Organization

Other

Registry information

Official study title

Nutritional Study:The Effect of Meat and Food Drived Polyphenols on Oxidize LDL Level

Important dates

Study start
2010
Primary completion
2011
Study completion
2011
First posted
Jul 8, 2011
Registry last updated
Jul 8, 2011

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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