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Completed

NCT Number: NCT04157556

The Effect of Maximal Exercise on MMP9 and TIMP1 and The Role of MMP9 -1562 C/T and TIMP1 372 T/C Polymorphisms

Matrix metalloproteinases (MMPs) play a critical role in the remodeling and regeneration of skeletal muscle. Studies which investigate exercise and effects of MMPs and tissue inhibitors of matrix metalloproteinases (TIMPs) are contradictory. Also the role of MMP9 -1562 C/T and TIMP1 372 T/C polymorphisms on exercise are unclear. In accordance with this conditions the hypothesis of the study are as follows:

1. Levels of basal serum MMP9 and TIMP1 are different between athletes (volleyball, basketball, handball) and sedentary people. 2. Levels of serum MMP9 and TIMP1 response to maximal exercise are different between athletes (volleyball, basketball, handball) and sedentary people . 3. Relationship between levels of serum MMP9 and TIMP1 response to maximal exercise are different between athletes (volleyball, basketball, handball) and sedentary people. 4. Levels of basal serum MMP9 and TIMP1 are related to MMP9 -1562 C/T and TIMP1 372 T/C polymorphisms. 5. Relationship between levels of serum MMP9 and TIMP1 response to maximal exercise and MMP9 -1562 C/T and TIMP1 372 T/C polymorphisms are different between athletes (volleyball, basketball, handball) and sedentary people.

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Key information

Conditions

MMP9 MMP9 -1562 C/T TIMP1 TIMP1 372 T/C

Age range

18 year–35 year

Sex eligibility

Male

Study type

Interventional

Phase

Not applicable

Primary location

Ege University Faculty of Sports Science

Izmir, Turkey (Türkiye)

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

Group of athletes:

  • Male basketball players who have been training regularly for at least last 3 months.
  • Male volleyball players who have been training regularly for at least last 3 months.
  • Male handball players who have been training regularly for at least last 3 months.

Group of sedentary people:

  • Male individuals with similar physical characteristics to the group of athletes
  • Male individuals who have not exercise regularly for at least last 3 months.

Exclusion criteria

(For two groups):

  • Smoking regularly
  • Drinking alcohol regularly
  • Using drug and antioxidant substance
  • Being obese,
  • Being sick or having disability

Treatment and study plan

Yo-Yo Intermittent Recovery Test Level 1

Other

The test is a standard test battery with progressively increasing speeds. After every 40-m run (2x20 m), active rest is performed by walking or jogging at a distance of 10 m (2x5 m). for 10 seconds. Participants are trying to adjust their speed according to incoming audio signals.The test is terminated when the participants can not reach the signal twice. The test ends in 10-20 minutes depending on the level of participants.

Reference Bangsbo, J., Iaia, F. M., & Krustrup, P. (2008). The Yo-Yo intermittent recovery test. Sports medicine, 38(1), 37-51.

Krustrup, P., Mohr, M., Amstrup, T., Rysgaard, T., Johansen, J., Steensberg, A., ... & Bangsbo, J. (2003). The yo-yo intermittent recovery test: physiological response, reliability, and validity. Medicine & Science in Sports & Exercise, 35(4), 697-705.

Primary outcomes

  1. Level of MMP9 and TIMP1

    Time frame: Yo-Yo Intermittent Recovery Test level 1 (the test performed until the subject is exhausted, the test lasts 10-20 minutes according to endurance capacity of the participants)

    The effect of acute maximal exercise on serum MMP9 and TIMP1 levels in athletes and sedentary people.

  2. MMP9 -1562 C/T, TIMP1 372 T/C polymorphisms

    Time frame: Yo-Yo Intermittent Recovery Test level 1 (the test performed until the subject is exhausted, the test lasts 10-20 minutes according to endurance capacity of the participants)

    The role of MMP9 -1562 C/T and TIMP1 372 T/C polymorphisms on the effect of acute maximal exercise on serum MMP9 and TIMP1 in athletes and sedentary people.

Secondary outcomes

  1. Level of indicators of muscle damage and inflammation and oxidative stress

    Time frame: Yo-Yo Intermittent Recovery Test level 1 (the test performed until the subject is exhausted, the test lasts 10-20 minutes according to endurance capacity of the participants)

    The relationship between primary outcome measures and indicators of muscle damage, inflammation and oxidative stress.

    Indicators to be determined: creatine kinase (CK), aspartate aminotransferase (AST), alanine aminotransferase (ALT), Interleukin-6 (IL-6), C- reactive protein (CRP), total antioxidant status (TAS), total oxidant status (TOS), nitric oxide (NO)

  2. Level of blood lipids and lipoproteins, glucose, urea, creatinine, hemogram

    Time frame: Yo-Yo Intermittent Recovery Test level 1 (the test performed until the subject is exhausted, the test lasts 10-20 minutes according to endurance capacity of the participants)

    The relationship between primary outcome measures and blood lipids and lipoproteins, glucose, urea, creatinine and hemogram

Sponsors and collaborators

Lead sponsor

Dokuz Eylul University

Other

Collaborators

  • Ege University

Registry information

Official study title

The Effect of Maximal Exercise on Matrix Metalloproteinase Enzyme and Its Inhibitor (MMP9 and TIMP1) and The Role of MMP9 -1562 C/T and TIMP1 372 T/C Polymorphisms

Important dates

Study start
2019
Primary completion
2020
Study completion
2020
First posted
Nov 8, 2019
Registry last updated
Apr 30, 2020

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.