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NCT Number: NCT05111717

The Effect of Desfluran On Global Dna Methylation

The purpose of this study is to demonstrate the molecular effect of desflurane on DNA by measuring the global DNA methylation level in patients under going lumbar spinal stenosis surgery under general anesthesia.

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Key information

Conditions

DNA Damage

Age range

18 year–65 year

Sex eligibility

All sexes

Study type

Observational

Primary location

Mugla Sıtkı Kocman University Training and research Hospital

Muğla, Turkey (Türkiye)

About this study

Forty patients scheduled for lumbar spinal surgery will include in the study. Peripheral venous blood samples will collect prior to the induction of anesthesia (T1) and after 120 minutes (T2). Anesthesia will maintaine with 40%-60% O2-air mixture and 6% desflurane. DNA will obtain from blood samples with the help of DNA isolation kit and global DNA methylation analysis will perform by ELISA method.

Who can participate

Healthy volunteers accepted: No

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • Ability to understand and willingness to sign an informed consent form
  • Elective lumbar spinal surgery that will take at least 120 minutes,
  • American Society of Anesthesiologists (ASA) physical status I and II
  • Be between the ages of 18 and 65

Exclusion criteria

  • Patients with ASA physical status III or IV disease
  • Smoking or alcohol consumption
  • Chronic systemic disease (diabetes mellitus,chronic pulmonary disorders, chronic kidney and liver disorders
  • recently received radiation, chemotherapy
  • malignancy
  • blood transfusion in operation
  • use of antioxidant supplements
  • have received general anesthesia in the last three months
  • Patients with known occupational exposures (operating room personnel, chemical plant workers

Treatment and study plan

Desflurane

Drug

Peripheral venous blood samplesof patients will collected at two time points as follows: before the premedication and the induction of anesthesia (T₁): 120 minutes after the beginning of anesthesia (T₂). Anesthesia maintenance will be provided with 40% O₂ and minimum alveolar concentration (MAC) in 60% air with 6% desflurane. DNA will be obtained from blood samples with the help of DNA isolation kit, and global DNA methylation analysis will performed by ELISA method.

Primary outcomes

  1. Global DNA methylation change 5 minutes before and 120 minutes after anesthesia induction in peripheral blood mononuclear cells

    Time frame: 5 minutes before induction of anesthesia ,120 minutes after the beginning of anesthesia

    The global DNA methylation level will be measured 5 minutes before and 120 minutes after anesthesia induction.

Sponsors and collaborators

Lead sponsor

Muğla Sıtkı Koçman University

Other

Registry information

Official study title

The Effect of Desfluran Administered in General Anesthesia On Global Dna Methylation

Important dates

Study start
2021
Primary completion
2021
Study completion
2021
First posted
Nov 8, 2021
Registry last updated
Mar 15, 2023

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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