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Completed

NCT Number: NCT05113914

The Age-related Changes of Energy Metabolism in Physically Fit Adult Males

The influence of age and physical fitness level on the level of energy metabolism and antioxidant capacity in adult males.

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Key information

Conditions

Healthy

Age range

18 year and older

Sex eligibility

Male

Study type

Interventional

Phase

Not applicable

Primary location

Poznan University of Physical Education

Poznan, 61-871, Poland

About this study

Over four hundred healthy, adult males of different age categories and physical fitness levels were included in the study. There is much research convincing that in athletes of various sports disciplines the level of energy metabolism, oxidative stress, and amino acid profiles during years of training undergoes sports adaptation. The study aimed to evaluate how age and physical fitness level affects the level of purine metabolites and metabolites of nicotinamide adenine dinucleotide, the level of antioxidant capacity, and amino acid profiles. Investigators hypothesized that there will be an age-related decline in most of the physiological indices, but significant differences will appear regarding the level of an individual's sports adaptation.

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • healthy
  • non-smoking
  • physically fit, including top-level athletes

Exclusion criteria

  • no reported history of a cardiopulmonary or renal diseases, and other chronic diseases
  • no major orthopedic injury or illness resulting in an inability to run
  • no medications that could affect cardiopulmonary functions
  • normal resting electrocardiogram
  • body mass index (BMI) below 30.0 kg·m-2
  • normal erythrocyte count and Hb content
  • no pathological states known of significantly elevated adenylate pool and concentration of ATP, e.g. sickle cell disease, diabetes, leukemia, sepsis, tuberculosis, meningococcal infection, or renal insufficiency

Treatment and study plan

Cardio-pulmonary exercise test

Diagnostic Test

An incremental running test was conducted on the treadmill. Initially, the exercise protocol included standing still for 3 min and walking for 3 min at a constant speed of 4 km/h. After this introductory part, the speed was increased to 8 km/h and then every 3 min by 2 km/h until the volitional exhaustion was reached. During all procedure oxygen consumption was measured with the use of a portable breath-by-breath ergospirometer.

Primary outcomes

  1. Cardio-respiratory measures

    Time frame: during the intervention

    Cardiopulmonary exercise test (CPET) was conducted while an incremental running test on the treadmill using a portable breath-by-breath ergospirometer and heart rate monitor.

Secondary outcomes

  1. Blood Lactate level

    Time frame: baseline and immediately after the intervention

    Lactate concentration was performed by the spectrophotometric enzymatic method.

  2. Plasma purine nucleotides

    Time frame: baseline and immediately after the intervention

    The plasma purine metabolites were estimated in plasma using high-performance liquid chromatography (HPLC) with UV-VIS detection.

    Measurements performed: Guanosine, Inosine, Adenosine, Hypoxanthine, Xanthine, Uric Acid, Allantoin, Adenine Nucleotides [adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP), inosine monophasphate (IMP)], Guanine Nucleotides [guanosine triphosphate (GTP), guanosine diphosphate (GDP), guanosine monophosphate (GMP)].

  3. Erythrocyte Purine Metabolites

    Time frame: baseline and immediately after the intervention

    The erythrocyte purine metabolites were estimated in erythrocytes using high-performance liquid chromatography (HPLC) with UV-VIS detection.

    Measurements performed: Adenine Nucleotides [adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP), inosine monophasphate (IMP)], Guanine Nucleotides [guanosine triphosphate (GTP), guanosine diphosphate (GDP), guanosine monophosphate (GMP)].

  4. Hypoxanthine-guanine phosphoribosyltransferase (HGPRT) activity

    Time frame: baseline and immediately after the intervention

    The HGPRT activity was estimated in whole blood and in erythrocytes using high-performance liquid chromatography (HPLC) with UV-VIS detection.

  5. Nicotinamide Adenine Dinucleotide (NAD) and its metabolites

    Time frame: baseline and immediately after the intervention

    Nicotinamide Adenine Dinucleotide (NAD) and its metabolites were estimated in plasma using mass spectrometric procedure.

    Measurements performed: Nicotinamide Adenine Dinucleotide (NAD+), Nicotinamide (NA), 1-Methylnicotinamide (MNA), 1-methyl-2-pyridone-5-carboxamide (M2PY), 1-methyl-4-pyridone-5-carboxamide (M4PY), Nicotinamide Mononucleotide (NMN), 4-pyridone-3-carboxamide ribonucleoside (4PYR)

  6. Total antioxidant status (TAS)

    Time frame: baseline

    Total antioxidant status (TAS) measurement was performed using commercially available ELISA immunoassay.

  7. Thiobarbituric-acid-reactive substances (TBARS)

    Time frame: baseline

    The measurement of thiobarbituric-acid-reactive substances (TBARS) was performed using commercially available ELISA immunoassay.

  8. Malondialdehyde (MDA)

    Time frame: baseline

    The measurement of malondialdehyde (MDA) was performed using commercially available ELISA immunoassay.

  9. Polyphenols

    Time frame: baseline

    The measurement of polyphenols was performed using commercially available ELISA immunoassay.

  10. Reduced glutathione (GSH)

    Time frame: baseline

    The measurement of reduced glutathione (GSH) was performed using commercially available ELISA immunoassay.

  11. Glutathione peroxidase (GPx) activity

    Time frame: baseline

    The measurement of glutathione peroxidase (GPx) activity was performed using commercially available ELISA immunoassay.

  12. Aminoacid profile

    Time frame: baseline

    The levels of 20 aminoacids were estimated in plasma using mass spectrometric procedure.

Sponsors and collaborators

Lead sponsor

Poznan University of Physical Education

Other

Registry information

Official study title

The Influence of Age and Physical Fitness Level on the Level of Energy Metabolism and Antioxidant Capacity in Adult Males

Important dates

Study start
2011
Primary completion
2019
Study completion
2019
First posted
Nov 9, 2021
Registry last updated
Nov 16, 2021

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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