Skip to main content
OpenTrials
Active, Not Recruiting

NCT Number: NCT07189130

The Acute Effect of Different Resistance Exercise Loads on Irisin and Brain-derived Neurotrophic Factor in Blood Serum and Plasma

The aim of this study is to investigate different resistance training loads on BNDF and irisin levels in platelets, blood serum and plasma.

Active, Not Recruiting

This study is active but is not currently recruiting participants.

Notify Me

Key information

Conditions

BDNF Healthy Young Adult Irisin Lactate Resistance Exercise Stroop Test

Age range

18 year–30 year

Sex eligibility

Male

Study type

Interventional

Phase

Not applicable

Primary location

University of Ljubljana, Faculty of Sport

Ljubljana, 1000, Slovenia

About this study

In this study, the investigators will examine how three resistance training protocols acutely affect BDNF levels (in serum, plasma, platelet count, and BDNF per platelet) and irisin levels in blood (plasma). The resistance training protocols will differ in intensity, rest intervals between sets and repetition execution. Blood samples will be collected from participants at four time points: before the start of the protocol, 5 minutes after completion of the final set, 1 hour after completion of the final set, and 3 hours after completion of the final set. In addition, blood lactate levels will be monitored at the same time points. Heart rate will also be recorded (immediately before the start of the protocol and 30 seconds after completion of the final set). A control protocol will also be implemented, in which blood will be collected at the same time points. Data will be collected across five visits to the kinesiology laboratory at the University of Ljubljana, Faculty of Sport. Each visit will last approximately 4 hours, except for the first visit where 1RM will be measured.

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • males between 18 and 30 years
  • moderate level of physical activity assessed with IPAQ

Exclusion criteria

  • injuries
  • cardiovascular diseases
  • respiratory diseases
  • psychiatric diseases
  • metabolic diseases
  • other neurodegenerative diseases

Treatment and study plan

Exercise: 80% 1 RM with 2 minutes break

Other

Participant will do resistance exercise at 80% 1RM with 2 minutes break, 4 sets of each exercise. Included exercises are lat pull, bench press and leg press.

Other names: resistance exercise

Exercise: 80% 1RM with1 minute break

Other

Participant will do resistance exercise at 80% 1RM with 1 minutes break, 4 sets of each exercise. Included exercises are lat pull, bench press and leg press.

Other names: resistance exercise

Exercise: accenuated eccentric exercise

Other

Participant will do accenuated eccentric resistance exercise at 80% 1RM in concetric part of movement and 110% 1RM in eccentric part of movement with 2 minutes break, 4 sets of each exercise. Included exercises are lat pull, bench press and leg press.

Control

Other

Timeline of events will be same as in exercise protocol (blood withdrawal and Stroop test), except participants will not do any kind of exercise protocol, instead they will be seating.

Primary outcomes

  1. plasma BDNF

    Time frame: pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise.

    BDNF will be determined in blood plasma with ELISA kit.

  2. Irisin

    Time frame: pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise.

    Irisin will be measured in blood plasma.

  3. serum BDNF

    Time frame: pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise.

    BDNF will be determined in blood serum with ELISA kit.

  4. Platelet count

    Time frame: pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise.

    Platelet count in blood will be done.

  5. BDNF per platelet

    Time frame: pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise.

    The amount of BDNF per platelet will be calculated.

Secondary outcomes

  1. Lactate

    Time frame: pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise.

    Lactate will be measured in blood.

  2. Stroop test

    Time frame: pre, 15 minutes post exercise.

    Stroop test will be used as an assessment of cognitive functions. The number of read word will be assessed.

Sponsors and collaborators

Lead sponsor

University of Ljubljana

Other

Registry information

Official study title

The Acute Response of Irisin and Brain-derived Neurotrophic Factor in Blood to Resistance Exercise Load

Important dates

Study start
2025
Primary completion
2025
Study completion
2026
First posted
Sep 23, 2025
Registry last updated
Dec 16, 2025

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

Related clinical trials

Published trials that share one or more normalized conditions with this study.