Study of Coronary Calcification in Subjects With Autosomal Dominant Familial Hypercholesterolemia Heterozygous

The CAC score has demonstrated very powerful predictive power, particularly in asymptomatic populations.

• The CAC score can:

1.1- Identify subjects with high cardiovascular risk According to prospective studies, it is estimated that a CAC score> 400 is a CHD equivalent, with 10-year event rates exceeding 20% in asymptomatic patients. Prospective studies in young patients with a family history of cardiovascular disease or dyslipidaemia showed a higher risk of cardiovascular disease in those with a CAC score of 0.

1.2- Identify subjects with low cardiovascular risk Regardless of the presence of risk factors, meta-analyses have repeatedly shown the high negative predictive power associated with a CAC = 0. The absence of calcified plaque presents an extraordinarily low risk at 10 years (1.1% at 1.7%) regardless of the number of risk factors Regardless of the presence of risk factors, meta-analyses have repeatedly shown the high negative predictive power associated with a CAC = 0 with an annual mortality rate of 0.87 compared to 1.92 in those with CAC between 1 and 10. Finally, recent studies have questioned the indication of a statin in non-HeFH patients with CAC = 0.

Non-calcified plaques are not, by definition, detected by CAC tests and patients with CAC = 0 may only exhibit soft, non-calcified plaques. The prevalence of these non-calcified plaques in high-risk patients with acute coronary syndrome is 5% . The prevalence in heHF patients is unknown.

• Description of the population to be studied and justification of their choice. Recruitment of patients with familial hypercholesterolemia will be carried out at the Cardiovascular Prevention Unit of the Pitié-Salpêtrière Hospital and in the Cardiology Department of Saint-Antoine Hospital. Patients will be included in the study when they come for their usual consultation or as part of their cardiovascular assessment in day hospitalization.
• Brief description of the product (s) or experimental act (s)

The actions and blood tests added by the research are as follows:

Imaging: Coronary CT angiography with injection of iodinated contrast medium Biology: Calculation of cholesterol burden Vitamin D and K, estradiol, Parathormone IL-1β, IL-6, IL-11, IL-17 TGFβ1 TNFα,Genomic and proteomic analyses: Osteopontin (OPN), Osteocalcin, Osteoprotegerin, Osteonectin Receptor activator of nuclear factor kappa-B ligand, Bone morphogenetic protein 2 4 and 7, Human Bone metabolism simplicat Matrix Gla Protein MGP

• Summary of foreseeable benefits and risks known to those who are suitable for research.

Individual risk

• Risks and physical constraints: patients will undergo a standard blood sampling for lipid levels and other biochemistry dosages and the realization of an arterial carotid and femoral ultrasound, as well as the coronary CT (with injection).
• Risks associated with the disease: there are no directly study-related risks of worsening of any previous condition in relation to the current pathology in the realization of this study. The increased cardiovascular risk associated with heFH is represented mostly by coronary artery disease which is related to the increased lifelong high cholesterol exposure.
• Risk of irradiation: study subjects wil lbe exposed to 2 - 5 mSv for the realization of both CAC score and coronary CT.
• Risk linked to the venous draining of 36 mL of blood all in all: pain, bruise, vagal faintness(malaise)
• Risks associated to the injection of iodized contrast agent: allergic reaction.

This pilot study will evaluate the prevalence of high CAC score in asymptomatic patients with heHF. This will be a first step in improving knowledge and treatment of heHF since using the CAC score, the investigators:

• Would identify the patients with increased risk to whom premature / aggressive interventions are recommended.
• Would validate a new non-invasive marker of the coronary damage in this heterogeneous population.

This project enters the wider frame of the premature ageing of the cardiovascular system with consequences on the development of cardiovascular complications such as the vascular calcifications. It was designed to highlight subclinical changes of the vascular tree to improve the treatment of the heHF and prevent the long-term complications of this disease. This project could help to identify new markers of myocardial and arterial dysfunction to propose an adapted prevention. The evaluation of the efficiency of medicine can be envisaged at the end of this study and will be encouraged by the strictly non invasive nature of the procedure as well as by its excellent reproducibility. It will also help to define strategies of prevention to improve the management of heHF.

The results will be broadcasted by means of scientific publications and of presentations in conferences or congress.

Inclusion criteria

Patients with a heterozygous form of familial hypercholesterolemia:

• Aged 35 to 60 years old.
• Asymptomatic.
• No sign of ischemia with ECG.
• No personal history of coronary heart disease.
• Treated or untreated by cholesterol lowering treatment.
• Prior clinical examination performed
• Beneficiary of a social protection scheme or beneficiary (excluding AME)
• Informed patient and signed consent form

Exclusion criteria

• Person under tutorship or curatorship, or unable to give consent
• Pregnancy, breastfeeding, woman of childbearing potential in the absence of effective contraception - a urine pregnancy test will be done in hospital on the day of the coroscanner
• Contraindication to CT or injection of iodinated contrast medium or injection of esmolol hydrochloride
• Technical counter-indication: patient diameter> 70 cm, weight> 250 kg
• Renal insufficiency (CL <60)
• Personal history of cardiovascular disease and myocardial infarction
• Type 2 diabetes or uncontrolled diabetes mellitus for more than 5 years
• Uncontrolled hypertension
• Atrial fibrillation, ventricular arrhythmia
• Participation in another interventional research involving the human person or being in the exclusion period following previous research involving the human person, if applicable