Study of Collagen IV and XIX in Bronchoalveolar Lavage, Pulmonary Aspiration and Bronchial Biopsies

Collagens are proteins present in all tissues. Besides their structural role, recent data showed that they were able to regulate many cellular functions. Many interactions occur between extracellular matrix macromolecules, especially collagen, and various cell types. These interactions can be controlled by peptides derived extracellular matrix macromolecules, called matrikines. Previous work from the investigators laboratory and others have shown that several C-terminal domain (NC1 domains) from basement membrane-associated collagens could regulate many cellular activities.

Lung is an organ particularly abundant in basement membranes. It is likely that various lung diseases may affect metabolism of basement membrane associated collagens. To the investigators knowledge, no study has focused on the expression of collagen XIX α1 chain and collagen IV chains α3 and α4 chains in lung and studied possible variations of expression in various pathophysiological situations.

The aim of this study are to:

* Study the presence of collagen IV and XIX (or fragments) in different types of sampling such as bronchoalveolar lavage, pulmonary aspiration and bronchial biopsies * Evaluate quantitative variations of expression of these collagen in different pulmonary diseases, especially chronic obstructive bronchopneumopathy, infectious pneumonia, pneumonitis or lung cancer.

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Key information

Conditions

Chronic Obstructive Bronchopneumopathy Chronic Disease Disease Attributes Infections Infectious Pneumonia Lung Cancer Lung Diseases Lung Diseases, Obstructive Lung Neoplasms Neoplasms Neoplasms by Site Pathologic Processes Pathological Conditions, Signs and Symptoms Pneumonia Pneumonitis Pulmonary Disease, Chronic Obstructive Respiratory Tract Diseases Respiratory Tract Infections Respiratory Tract Neoplasms Thoracic Neoplasms

Age range

18 year and older

Sex eligibility

All sexes

Study type

Observational

Primary location

Chu Reims

Reims, 51092, France

About this study

Experimental Design: Cross-sectional study, with prospective inclusions, single center.

Investigations:

Realization of bronchoscopy according to the indications (with or without bronchoalveolar lavage with or without bronchial biopsy, participation in research that do not modify patient's care)
samples are sent to the laboratory, centrifuged and stored at -80°C until they were assessed.
samples were used to specific analysis in this study:
Study of qualitative expression of collagen IV and XIX by Reverse transcription polymerase chain reaction (RT-PCR) and Western Blot
Study of quantitative variations of these collagens by ELISA

Statistical analysis planification:

description of the data (mean and standard deviation),
comparison of different groups (non-pathological vs pulmonary disease) by Student's t test, Wilcoxon, Chi2 or Fisher test.

Expected results and prospects:

The investigators should be able to demonstrate the presence of collagen IV and/or collagen XIX or different fragments in biological samples. The investigators would then be able to show variation in expression of these collagens according to different pathological situations. In the longer term, the objective is to validate these collagens as a markers of pulmonary pathologies or as therapeutic agents.

Who can participate

Healthy volunteers accepted: No

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

Patient from the division of pulmonary medicine of the Reims University Hospital
Patient that must undergo a bronchoscopy with bronchoalveolar lavage
Male or female
Informed of the study (written information) and not objecting to it (oral agreement)

Exclusion criteria

Aged bellow 18 years

Treatment and study plan

biological fluids (serum, bronchoalveolar lavage and pulmonary aspiration) and bronchial biopsies collection

Biological

Primary outcomes

Collagen expression

Time frame: 9 months

western blot and Reverse transcription polymerase chain reaction
Collagen expression

Time frame: 15 months

western blot and Reverse transcription polymerase chain reaction

Sponsors and collaborators

Lead sponsor

CHU de Reims

Other

Registry information

Acronym: BRONCOLL

Important dates

Study start: 2014
Primary completion: 2017
Study completion: 2017
First posted: Jun 2, 2016
Registry last updated: May 13, 2021

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

Published trials that share one or more normalized conditions with this study.

Completed