Serum Kisspeptin Levels in Infertile Women

The study population is comprise of 90 women those age varies between 18-38 year-old with wish of baby for at least one year despite of unprotected sexual intercourse, patent tubes confirmed in hysterosalpingography , follicle stimulating hormone levels <12 IU. The first group will comprise of 30 women with polycystic ovaries in ultrasound examination or anovulatory cycles, the second group will comprise of 30 women with diagnosis of unexplained infertility, normal menstrual periods and without known male factor infertility and the third group will comprise of 30 women those partners with sperm count between 5x106 -15x106 /mL, type A + type B motility <%32 and Kruger morphology <4%. A 2 cc blood sample for Kisspeptin analysis will be drawn from the antecubital vein from each participants and centrifuged and sera will be kept in -80 C till recruitment has been completed. Kisspeptin, E2, Progesterone, anti-mullerian hormone levels will be measured with ELISA method. The patients will receive 50 mg clomiphene citrate at the fifth day of the menstrual period and follicular development will be measured with serial ultrasound follow up.

Inclusion criteria

• ages varying between 18-38 year-old with wish of baby for at least one year despite of unprotected sexual intercourse
• patent tubes confirmed in hysterosalpingography
• follicle stimulating hormone levels <12 IU
• no other relevant medical history

Exclusion criteria

• ages other than 18-38 year-old with wish of baby for least than one year despite of unprotected sexual intercourse
• obstructed tubes confirmed in hysterosalpingography
• follicle stimulating hormone levels >12 IU
• history thyroid disease
• increased prolactin levels
• history of chemo/radiation therapy