Selection of Resistant Mutations to Dolutegravir in PLWH Treated in Mozambique

This research study consists of a single visit, in which you will be taken an additional vial of blood to analyze the presence or absence of mutations in your virus to HIV drugs. In addition, other HIV infection routine information will be recorded:

• Demographic data
• HIV infection history data
• Virological failure information
• Laboratory parameters Resistance tests techniques: Genotyping resistance test will be performed from plasma samples in those patients with viral load over 200 copies/ml, using ultracentrifugation if needed (Inzaule, Seth C et al. J Antimicrob Chemother, 2018). Briefly, RNA will be extracted and HIV-1 pol gene will be amplified, followed by a nested-PCR. Amplification products will be purified, quantified and finally diluted and prepared for sequencing following Illumina DNA Sample Preparation Kit protocol (Illumina, San Diego, CA). Samples will finally be pooled and sequenced in a MiSeq System (Illumina). MiSeq sequences will be downloaded from Basespace (Illumina) and analysed using PASeq (IrsiCaixa,Barcelona,ES), v1.4.18 (https://www.paseq.org). The questionnaire about clinical and demographic characteristics will be performed during the study visit

Inclusion criteria

• Patients able to understand and sign the informed consent form.
• Documented HIV-1 infection.
• Under the first line TLD for at least 6 months.
• At least 3 months follow-up with CV >200c/ml.

Exclusion criteria

• Patients <18 years of age.
• Under the first line regime other than TLD.
• Patients with active neoplastic processes.
• Patients with active opportunistic diseases.
• Patients unable to give informed consent.