Screening Nasopharyngeal Carcinoma With EBV Associated Biomarkers in Zhongshan City

Sample selection

• Select communities of 20,000 to 40,000 populations in Zhongshan City and Wuzhou city as the investigators' fields.

Participants recruitment

• Subjects voluntarily attend this screening study Informed consent - Informed consent forms will be collected at the recruitment. Questionnaires.
• Face-to-face interviews are conducted by well-trained investigators.

Tests and follow up

• At initial screening, all participants will be tested for EBV associated biomarkers, including EBNA1-IgA, VCA-IgA, P85-Ab et al. And in males, EBV-DNA will be tested. Based on EBNA1-IgA, VCA-IgA, participants will be stratified into high, moderate and low risk. Screening positive include P85-Ab positive, high or moderate risk of NPC or EBV DNA positive. The rest will be screening negative.
• During the initial screening and following up, people with high risk or P85-Ab positive will refer to the diagnostic workup for NPC. Briefly, fiberoptic endoscopy and pathological biopsy will be performed by otorhinolaryngologists.
• Screening positive people not diagnosed as nasopharyngeal carcinoma in the first year will be followed up annually.
• EBV DNA single positive people will refer to the diagnostic workup for NPC in the third year of follow up.
• The rest of the blood samples will be stored at the biobank of Zhongshan People's Hospital for further research.
• All subjects will also be followed by record linkage to Cancer Register and Population Register.

Inclusion criteria

• Subject residents in Zhongshan City or Wuzhou City
• Subject has no medical record of nasopharyngeal carcinoma
• ECOG 0-2
• Subject is able to comprehend, sign, and date the written informed consent document to participate in the study.
• Subject has psychical condition and well consciousness, and also accept and cooperate with the follow-up of this study.

Exclusion criteria

• Subject has heavy cardiovascular, liver or kidney disease.
• Subject has contraindications to nasopharyngeal fiberoptic endoscopy.