Role of Mitochondrial Bioenergetics in Resilience for Gynecologic Cancer

Proportion of approached patients who signed consent out of the total number of patients who were approached

Proportion of consented participants who initiated at least one pretreatment assessment or had pretreatment blood drawn out of the total number of consented participants

Proportion of enrolled participants who completed at least one posttreatment assessment (cognitive or physical) out of the total number of enrolled participants

≥80% enrollment rate, ≥80% completion rate (30 of 37)

The PROMIS Fatigue 7a is a validated seven-item questionnaire assessing fatigue severity and its impact on daily activities during the past 7 days. Each item is rated on a 5-point scale. Raw scores range from 7 to 35, with higher scores indicating greater fatigue.

The SPPB is a measure of lower-extremity physical function consisting of three components: walking speed, repeated chair stands, and standing balance in three increasingly difficult positions.

Walking speed is assessed by asking the participants to walk at their usual pace over a 4-meter course. Walking speed is scored from zero to four, with four indicating the highest level of performance and zero indicating an inability to complete the task.

The time to complete the task will be recorded. Repeated chair stands is scored from zero to four, with four indicating the highest level of performance and zero indicating an inability to complete the task.

For the test of standing balance, participants are asked to maintain balance in three positions, characterized by a progressive narrowing of the base of support: feet together (side-by-side), the heel of one foot beside the big toe of the other foot (semi-tandem), and the heel of one foot in front of and touching the toes of the other foot (tandem). For each of the three positions, participants are timed to a maximum of 10 seconds. Standing balance is scored from zero to four, with four indicating the highest level of performance and zero indicating an inability to complete the task.

Handgrip strength is a fundamental measure of upper body muscle strength and overall physical fitness.

Raw scores from the three learning trials are combined into a total recall score. The number of recalled words during the delayed recall portion of the test is the delayed recall score. The retention score (%) is the result of the delayed recall score divided by the highest raw score from learning trial #2 or learning trial #3.

TMT-A measures psychomotor speed, visuospatial search, and target-directed motor tracking.

TMT-B measures executive functions such as cognitive flexibility and set-shifting.

The COWAT measures the speed of lexical access, which is the ability to retrieve the meaning and pronunciation of a word from one's mental dictionary.

Participant is shown 31 color photographs of objects. The participant is allowed 10 seconds to recall the name of each object.

Maximum oxygen consumption rates from mitochondrial complexes I and II are measured by adding specific substrates that activate each complex in PBMCs

Maximum oxygen consumption rates from mitochondrial complexes I and II are measured by adding specific substrates that activate each complex in muscle tissue

Participant recalls sequences of numbers in forward and backward order. Sequences are read at a rate of 1 digit per second and increase in length until the participant fails two consecutive sequences of the same length.

Protein expression related to electron transport chain activity-including components of the Krebs cycle, fatty acid oxidation, and antioxidant pathways-was measured using proteomics in PBMCs

Protein expression related to electron transport chain activity-including components of the Krebs cycle, fatty acid oxidation, and antioxidant pathways-was measured using proteomics in muscle tissue