Retinal Blood Flow and Microthrombi in Type 1 Diabetes

The ultimate goal of this research is to contribute to the development of strategies to prevent diabetic retinopathy. This project will test the hypothesis that antiplatelet agents normalize the reduced blood flow observed early in the course of type 1 diabetes. If the hypothesis is proven correct, the results will indicate that the formation of small blood clots (microthrombi) occurs early in diabetic retinal vessels. In turn, because microthrombosis could readily account for the occlusive microangiopathy that causes the sight-threatening stages of diabetic retinopathy, the results will propose the desirability of antiplatelet therapy for the prevention of diabetic retinopathy.

We have three specific aims:

• To confirm that, under basal conditions, retinal blood flow measured with the laser Doppler method in a group of type 1 diabetic patients with no or minimal retinopathy differs from the flow measured in age- and sex-matched nondiabetic control subjects;
• To determine whether the response of retinal blood flow to low-dose aspirin (81 mg/day) administered for 2 months versus placebo, differs between type 1 diabetic patients with no or minimal retinopathy and age- and sex-matched nondiabetic control subjects;
• To determine whether in type 1 diabetic patients with no or minimal retinopathy the response of retinal blood flow to low-dose aspirin differs from the response to clopidogrel, a drug that interferes with platelet function downstream of the site of aspirin action.

Inclusion criteria

• Age 18-45 years,
• type 1 diabetes duration 1-15 years,
• absent or minimal retinopathy (EDTRS 20).
• Age- and gender-matched healthy controls

Exclusion criteria

• smoking,
• systemic diseases other than diabetes,
• retinal diseases other than diabetic retinopathy,
• pregnancy,
• bleeding disorders,
• aspirin allergy,
• use of anti-platelet agents,
• non-steroidal anti-inflammatory agents,
• angiotensin converting enzyme inhibitors,
• angiotensin receptor antagonists,
• Vitamin E in large doses.