Repeatability and Agreement of Anterior Segment Optical Coherence Tomography (AS-OCT) and Thermography

Study Objectives and Purpose:

Part A - AS-OCT repeatability and agreement

• The primary purpose of Part A is to compare the repeatability of the Cirrus with the Visante AS-OCT imaging system.
• The second objective is to identify the level of agreement in the tear meniscus measurements taken by the Cirrus and Visante AS-OCT.

Part B - Tear evaporimetry with functional infra-red thermography

• To evaluate the repeatability of Infrared thermography for ocular surface temperature measurements that are used for calculation of tear evaporation rates. If this is found to be acceptable, it will be correlated to tear meniscus parameters.
• To determine whether this method is able to detect temperature changes of the ocular surface.

Study design:

Part A. Cross sectional study. Part B. Prospective study

Rationale:

Part A - AS-OCT repeatability and agreement Repeatability is an important issue in the objective assessment of dry eye. While previous studies have compared various methods of measuring the tear meniscus, including the use of Visante AS-OCT, current literature lacks studies that investigated the Cirrus AS-OCT for assessment of dry eye. With a higher resolution, the Cirrus is potentially useful as a non-invasive procedure for objective measurements of the tear meniscus.

Part B - Tear evaporimetry with functional infra-red thermography Current available methods to measure of tear evaporation rate have major limitations. This new method, however, is non-invasive, has no need for special eye drops or chambers and takes temperature measurements very quickly. This method is therefore extremely practical and provides many exciting possibilities for future trials.

Methods:

Participants and target sample size 40 volunteers from Singapore Eye Research Institute will be recruited for this study. Each volunteer will be given a study serial number to ensure anonymity.

20 will participate in the AS-OCT study. 20 will participate in the tear evaporimetry study.

Part A - AS-OCT repeatability and agreement Two AS-OCT imaging systems, namely Cirrus and the Visante, will be tested. All participants undergo acquisition of 2 images by the Visante AS-OCT, then 2 images by the Cirrus AS-OCT. Both images will be acquired by the same technician.

Part B - Tear evaporimetry with functional infra-red thermography In total, four pairs of thermographic sequences on the ocluar surface temperature from volunteers will be taken.

• The volunteer will be seated in front of the thermographic camera and on each eye on thermographic sequence will be captured.
• After 20 minutes, a second pair of thermographic sequences will be captured.
• An eye mask with a temperature of not more than 40 deg C (confirmed by thermometer) will be worn by the volunteer for 5 minutes.
• A third pair of thermographic sequences will be captured immediately after mask removal.
• A fourth pair of thermographic sequences will be captured 1 hour after mask removal.

Between sequence acquisitions, the volunteer is allowed to move freely in the room. The temperature and humidity of the room will be kept constant during measurements.

Visit schedules:

Only one examination per ASOCT instrument for Part A is required. For Part B, all thermography will be performed on the same day.

If participants agree to have both ASOCT and tear evaporimetry done, then they will undergo Parts A and B. If not, they will be recruited for either Part A or Part B.

Duration of study: 1 day

Inclusion criteria

• Volunteers that are medically fit and willing to participate in this study.

Exclusion criteria

• No eye surgery done within the previous 3 months
• Active ocular infection or pterygium that may affect tear film stability.
• Any other specified reason as determined by clinical investigator.