Relationship Between Skin Microbiome, Epigenetic Age, and Facial Features in Aging

Inclusion criteria

• Healthy subject;
• Sex: female
• Age: 50% having between 25 to 35 years old or 50% between 55 to 65 years old
• Type: Caucasian
• Phototype: I to III on the Fitzpatrick scale
• Subject, having given freely and expressly her informed consent.
• Subject, psychologically able to understand the study related information and to give a written informed consent;
• Subject able to comply with protocol requirements;
• Female of childbearing potential should use a medically accepted contraceptive regimen since at least 12 weeks before the beginning of the study and during all the study.
• Women between 55 to 65 years old should be menopausal (last menstrual period at least 12 months before the screening visit

Exclusion criteria

• Pregnant or nursing woman or planning a pregnancy during the study;
• Subject who had been deprived of their freedom by administrative or legal decision or who is under guardianship;
• Subject who is under guardianship or who is not able to express her consent;
• Subject in a social or sanitary establishment;
• Subject suspected to be non-compliant according to the investigator's judgment;
• Subject having received 6000 euros indemnities for participation in a clinical research in the 12 previous months, including participation in the present study;
• Subject enrolled in another clinical trial or which exclusion period is not over.
• Subject with a condition or receiving a medication which, in the investigator's judgment, put the subject at undue risk or which may interfere with the evaluation;
• Subject suffering from a severe or progressive disease.
• Subject with current cutaneous inflammatory or infectious processes (eg, acne, herpes), abscess, an unhealed wound, or a cancerous or precancerous lesion on the face that could interfere with the evaluation.
• Subject with a tattoo, a scar, moles, acne, too many hairs or anything on the studied zones which might interfere with the evaluation.
• Subject having received injection on the face with botulinum toxin in the year before the screening.
• Subject having received injection of filling product on the face in the year before the screening visit.
• Subject having received injection of mesotherapy in the face in the past 6 months before the screening visit.
• Subject having done a superficial or medium peeling or a superficial scrub on the face within the 2 previous months.
• Subject having received tensor threads on the face in the two years before the screening visit.
• Subject having received treatment with a laser, ultrasound or radiofrequency treatment, a dermabrasion, a surgery, a chemical peeling or any other procedure based on active dermal response on the face within the past 6 months.
• Subject having used LED mask in the three weeks before screening visit
• Subject undergoing a topical treatment on the face or a systemic treatment:

• anti-inflammatory medication and/or anti-histamines during the previous 2 weeks and during the study;
• corticosteroids during the 2 previous weeks and during the study;
• retinoids, anti-acne treatment and/or immunosuppressors during the 3 previous months and during the study;
• any medication stabilized for less than one month.
• Subject having started or changed her oral contraceptive treatment during the three previous months.
• Subject under oral hormonal substitutive treatment during the year previous the screening visit
• Intensive exposure to sunlight or UV-rays within the previous month and/or foreseen during the study;
• Subject having changed her life habits within the previous month and/or during the study;
• Subject with an excessive consumption of alcohol (more than 2 glasses of wine per day) and/or tobacco (more than 10 cigarettes per day).
• Subject having started or changed her face care products and or make-up within the previous month