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Completed

NCT Number: NCT02873988

Regeneration of Bronchial Epithelium During Chronic Obstructive Pulmonary Disease

Airway epithelium integrity is essential to maintain its role of mechanical and functional barrier. Recurrent epithelial injuries require a complex mechanism of repair to restore its integrity. In chronic obstructive pulmonary disease (COPD), an abnormal airway epithelial repair may participate in airway remodelling. The objective was to determine if airway epithelial wound repair of airway epithelium is abnormal in COPD.

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Key information

Conditions

COPD Chronic Disease Disease Attributes Lung Diseases Lung Diseases, Obstructive Pathologic Processes Pathological Conditions, Signs and Symptoms Pulmonary Disease, Chronic Obstructive Respiratory Tract Diseases

Age range

40 year and older

Sex eligibility

All sexes

Study type

Observational

Primary location

Chu de Reims

Reims, France, 51092

About this study

Patients scheduled for lung resection were prospectively recruited. Demographic, clinical data and pulmonary function tests results were recorded. Emphysema was visually scored and histological remodelling features were noted. Primary bronchial epithelial cells (BEC) were extracted and cultured for wound closure assay. The investigators determined the mean speed of wound closure (MSWC) and cell proliferation index, matrix metalloprotease (MMP)-2, MMP-9 and cytokines levels in supernatants of BEC 18 hours after cell wounding. In a subset of patients, bronchiolar epithelial cells were also cultured for wound closure assay for MSWC analyze.

Who can participate

Healthy volunteers accepted: No

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

6 Patients scheduled for lung resection for cancer

Exclusion criteria

  • Patients with asthma, cystic fibrosis, bronchiectasis or pulmonary fibrosis were excluded

Treatment and study plan

bronchial epithelial cell culture

Other

Primary outcomes

  1. Mean speed of wound closure (MSWC) of wounded bronchial epithelial cell culture obtained from COPD and control patients

    Time frame: hour 18

Secondary outcomes

  1. Cell proliferation index in supernatants of bronchial epithelial cells 18 hours after cell wounding.

    Time frame: hour 18

  2. Matrix metalloprotease (MMP)-2 level in supernatants of bronchial epithelial cells 18 hours after cell wounding.

    Time frame: hour 18

  3. Matrix metalloprotease (MMP)-9 level in supernatants of bronchial epithelial cells 18 hours after cell wounding.

    Time frame: hour 18

  4. Cytokines levels in supernatants of bronchial epithelial cells 18 hours after cell wounding.

    Time frame: hour 18

Sponsors and collaborators

Lead sponsor

CHU de Reims

Other

Registry information

Acronym: EPITHREGENCOPD

Important dates

Study start
2012
Primary completion
2013
Study completion
2014
First posted
Aug 22, 2016
Registry last updated
Aug 22, 2016

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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