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Completed

NCT Number: NCT06260215

Recovery Kinetics Following a Soccer Training in Middle-aged Males

This study aims at investigating the recovery kinetics of skeletal muscle damage, neuromuscular fatigue and performance following a single soccer trainning session in middle aged males. The participants will perform a soccer training session [A single training session including 60 minutes of warm up, soccer technical exercises and small-sided game] and a cotrol trial (No intervention included, only daily measurements) in randomized, repeated measures, crossover design. Assesesments related to skeletal muscle damage, performance and neuromuscular fatigue will be executed before the training session and daily for four consecutive days after training.

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Key information

Conditions

Skeletal Muscle Damage Inflammation Pathologic Processes Pathological Conditions, Signs and Symptoms

Age range

40 year–60 year

Sex eligibility

Male

Study type

Interventional

Phase

Not applicable

Primary location

University o Thessaly, School of Physical Education and Sports Science

Trikala, 42100, Greece

About this study

Eleven male middle-aged participants will be included in this study. Participants will be initially informed about the main goals of study as well as the associated risks and benefits and then will provide their signed consent form. At baseline, they will undergo assessment of their anthropometrics (body mass, height, BMI, WHR), body compotition (using DXA), physical contition level (peak oxygen consumption, VO2peak), haemodynamic profile (diastolic and diastolic blood pressure) and daily dietary intake.Thereafter, they will participate in two experimental trials (Control trial and Soccer trial) in a randomized, crossover, repeated measure design: 1) ST (Soccer Trial): Participants in this trial will perform a soccer training session (60 min) consisted of warm-up, technical exercises and small-sided game, CT (Control Trial): Participants in this trial will only execute the evaluations daily in the dependent variables (they will not receive any intervention). Before each trial, the participants will provide a resting blood sample (for the determination of white blood cells, granulocyte cells, monocyte cells, lymphocyte cells, Hemeatocrit, Hemoglobin, red blood cells and platelets, total antioxidant capacity, glutathione and creatine kinase activity) and undergo assessments of their delay onset muscle soreness (DOMS), maximal voluntary isometric contraction (MVIC) of knee extensors and flexors (by isokinetic dynamometer), height and power of countermovement jump (CMJ-using force platform), 10m and 30m sprint time (will be evaluated using light cells). A 7-day wash-out period will apllied between ST and CT. The blood sample will be collected before and 24, 48 and 72 hours post-taining. Also, the evaluation of DOMS, MVIC, sprinting performance, power and jump height (by CMJ) will be evaluated before and 24, 48, 72 and 96 hours post training.

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • Free of musculoskeletal injuries
  • No use of ergogenic supplements or medication
  • Free of chronic diseases
  • age of 40-60 years

Exclusion criteria

  • Musculoskeletal injury
  • Use of alcohol, caffeine and any type of ergogenic supplements or medication during the course of the study

Treatment and study plan

ST

Other

Participants will perform a soccer training (ST) session for 60 minutes. The training will include the warm-up, soccer technical exercises and a small-side game

Primary outcomes

  1. Change in White blood cell count

    Time frame: At baseline, at 24, 48 and 72 hours after soccer training

    White blood cell count will be measured using an automatic blood analyzer

  2. Change in Granulocyte cell count

    Time frame: At baseline, at 24, 48 and 72 hours after soccer training

    Change in White blood cell count will be measured using an automatic blood analyzer

  3. Change in Platelets cell count

    Time frame: At baseline, at 24, 48 and 72 hours after soccer training

    Platelets cell count will be measured using an automatic blood analyzer

  4. Change in Monocyte cell count

    Time frame: At baseline, at 24, 48 and 72 hours after soccer training

    Monocytes cell count will be measured using an automatic blood analyzer

  5. Change in Lymphocyte cell count

    Time frame: At baseline, at 24, 48 and 72 hours after soccer training

    Lymphocyte cell count will be measured using an automatic blood analyzer

  6. Change in percentage of Hematocrit level

    Time frame: At baseline, at 24, 48 and 72 hours after soccer training

    The percentage of Hematocrit level will be measured using an automatic blood analyzer

  7. Change Red blood cells count

    Time frame: At baseline, at 24, 48 and 72 hours after soccer training

    Red blood cells count will be measured using an automatic blood analyzer

  8. Change in Hemoglobin level

    Time frame: At baseline, at 24, 48 and 72 hours after soccer training

    Hemoglobin level will be measured using an automatic blood analyzer

  9. Change in Creatine kinase activity

    Time frame: At baseline, at 24, 48 and 72 hours after soccer training

    Creatine kinase activity will be measured in plasma

  10. Change in Total antioxidant capacity

    Time frame: At baseline, at 24, 48 and 72 hours after soccer training

    Total antioxidant capacity will be measured in plasma

  11. Change in Glutathione concentration in blood

    Time frame: At baseline, at 24, 48 and 72 hours after soccer training

    Glutathione concentration will be measured in red blood cells

  12. Change in countermovement jump height

    Time frame: At baseline, at 24, 48, 72 and 96 hours after soccer training

    countermovement jump height will be measured using a force platform

  13. Change in Peak power during coutermovement jump test

    Time frame: At baseline, at 24, 48, 72 and 96 hours after soccer training

    The peak power will be measured using a force platform

  14. Change in isometric peak torque of knee extensors and flexors

    Time frame: At baseline, at 24, 48, 72 and 96 hours after soccer training

    Isometric peak torque will be assessed on an isokinetic dynamometer

  15. Change in sprint time of 10 m

    Time frame: At baseline, at 24, 48, 72 and 96 hours after soccer training

    10m sprint time will be assessed using light cells

  16. Change in sprint time of 30 m

    Time frame: At baseline, at 24, 48, 72 and 96 hours after soccer training

    30m sprint time will be assessed using light cells

  17. Change in delayed onset of muscle soreness

    Time frame: At baseline, at 24, 48, 72 and 96 hours after soccer training

    Muscle soreness will be assessed by palpation of muscle belly and the distal region

Secondary outcomes

  1. Dietary intake

    Time frame: At baseline

    Dietary intake will be assesed over a 7-day period using diet recalls

  2. Peak Maximal oxygen consumption (Peak VO2)

    Time frame: At baseline

    Peak VO2 will be estimated by open circuit spirometry via breath by breath method

  3. Body Mass

    Time frame: At baseline

    Body Mass will be measured on a beam balance with stadiometer

  4. Body Height

    Time frame: At baseline

    Body Height will be measured on a beam balance with stadiometer

  5. Body Fat

    Time frame: At baseline

    Body Fat will be measured by using Dual-emission X-ray absorptiometry

  6. Lean body mass

    Time frame: At baseline

    Lean body mass will be measured by using Dual-emission X-ray absorptiometry

  7. Bone mass density

    Time frame: At baseline

    Bone mass density will be measured by using Dual-emission X-ray absorptiometry

  8. Bone mass content

    Time frame: At baseline

    Bone mass content will be measured by using Dual-emission X-ray absorptiometry

  9. Field activity during the soccer training

    Time frame: During the 60 minutes soccer training (ST) session (72 hours after baseline measurements)

    The field activity will be continuously monitored during the soccer training using global positioning system (GPS)

  10. Heart rate during the soccer training

    Time frame: During the 60 minutes soccer training (ST) session (72 hours after baseline measurements)

    Heart rate will be continuously monitored during the soccer training using heart rate monitors

  11. Systolic Blood Pressure

    Time frame: At baseline

    Systolic Blood Pressure will be examined using sphygmomanometer

  12. Dystolic Blood Pressure

    Time frame: At baseline

    Dystolic Blood Pressure will be examined using sphygmomanometer

  13. Rest Heart Rate

    Time frame: At baseline

    The rest heart rate will be estimated using a heart rate monitor

Sponsors and collaborators

Lead sponsor

University of Thessaly

Other

Registry information

Important dates

Study start
2024
Primary completion
2024
Study completion
2024
First posted
Feb 15, 2024
Registry last updated
Dec 2, 2025

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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