PTCs-based Precision Treatment Strategy on Recurrent High-grade Gliomas

The investigators have established a precision treatment strategy, that select chemotherapeutic drugs or targeted drugs based on information from PTCs drug-screening or/and bioinformatic prediction. In this study, the investigators are going to exploit this strategy for the precision treatment of recurrent high-grade gliomas. The investigators will evaluate the feasibility, safety and preliminary efficacy via collecting the indexes comprising clinical presentation, results of imaging examination, clinical assays, KPS, neurological score, etc.

Inclusion criteria

• 1 At the age of 18~75, regardless the gender
• 2 The lesion of primary surgery was diagnosed as WHO II~IV Glioma by histologic pathology
• 3 Received radiotherapy and Temozolomide-based chemotherapy within 5 years
• 4 Recurrent and respectable gliomas, and have been neurosurgically resected
• 5 The resected recurrent gliomas was identified as WHO III~IV Glioma by histologic pathology
• 6 None postoperative standard therapeutic regimens can be followed when participating the recruitment
• 7 Can understand the trial's content and sign informed consent

Exclusion criteria

• 1 Having other untreated malignant tumors
• 2 The amount of resected tumors is not enough for genomic sequencing or PTCs drug screening
• 3 Received Carmustine implants within 6 months prior to enrollment
• 4 Subjects with active HBC, HCV or HIV infection
• 5 Subjects with uncontrolled cardio- or cerebro- vascular diseases
• 6 Subjects with uncontrolled psychiatric diseases or condition that could increase adverse events or interfere the evaluation of outcomes
• 7 Subjects with other conditions in their active phase that would interfere trial participation
• 8 Subjects receiving immunosuppressants after organ transplantation
• 9 Subjects with unstable pulmonary embolism, deep venous embolism, or other major arterial and venous thromboembolic events that occur within 30 days before the enrollment, or receiving anticoagulant therapy
• 10 Subjects in pregnancy or breastfeeding, or those who plan to become pregnant during treatment or within 2 months after the end of treatment
• 11 Subjects with other conditions that would interfere participating in the trial at the investigator's discretion
• 12 Subjects with medical conditions that affect signing the written informed consent or complying with the research procedures; or patients who are unwilling or unable to comply with the research procedures