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NCT Number: NCT06431334

Protein Requirements Amongst Male Masters-Level Athletes Following a Cycling Exercise Bout as Determined by the Indicator Amino Acid Oxidation Technique

Masters level cyclists are a population above the age of 35 years who frequently participate in prolonged as well as heavy-volume training. Like most endurance-trained athletes, a greater recommended dietary allowance (RDA) for protein of 1.2-1.4 g/kg/bw is suggested.

Dietary protein intake is vital for maximizing the benefits of training and ensuring optimal recovery. Dietary recommendations traditionally have been determined through nitrogen balance techniques, however, recent research indicates how this method is potentially underestimating protein requirements. Therefore, there is a need to reassess current dietary recommendations in order to meet the demands of physical activity for highly active populations.

Recent efforts to understand protein requirements during rest and following exercise have been completed using the indicator amino acid technique (IAAO). This non-invasive method is reported to provide a robust measure of protein requirements. However, there is limited work in older (≥60 years) active populations.

The purpose of this study is to measure the protein requirements in master cyclists, following an endurance training session, using the non-invasive IAAO technique.

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Key information

Conditions

Healthy Dietary Reference Intakes Increased Metabolic Requirements

Age range

65 year and older

Sex eligibility

Male

Study type

Interventional

Phase

Not applicable

Primary location

McGill University

Montreal, Quebec, H3A 0G4, Canada

Location status: Recruiting

Location contact

Damian Papadopoulos, B.Sc.

CONTACT

[email protected]

5145617860

Damian Papadopoulos, BSc

SUB_INVESTIGATOR

Tyler Churchward-Venne, PhD

CONTACT

[email protected]

(514) 399-9684

Tyler Churchward-Venne, PhD

PRINCIPAL_INVESTIGATOR

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • 18.5< BMI <35 kg/m^2
  • Overtly healthy masters level cyclists ≥65 years
  • Masters level cyclist training (>4 days per week; ~100 km per week)
  • Body mass stable over previous 6-months
  • Availability for multiple metabolic trials (~7 trials)
  • Ability to travel to and from laboratory facility

Exclusion criteria

  • Recent history of weight loss or weight gain (>5% body weight)
  • Uncontrolled hypertension
  • ≥2 chronic diseases (e.g. diabetes, osteoporosis, dyslipidemia)
  • Acute illness that could affect protein metabolism (HIV, renal dysfunction, influenza)
  • Currently using anti-inflammatory medications
  • Inability to adhere to study protocol (i.e., alcohol, caffeine, dietary restrictions)
  • Regular tobacco user
  • Illicit drug use
  • Habitually ingesting ≥3 g/kg/bw

Treatment and study plan

Amino acid intake

Dietary Supplement

Amino acid intakes will vary between 0.2 to 2.8 g/kg/d

Primary outcomes

  1. 13CO2 Excretion

    Time frame: 7-weeks

    Measured by continuous-flow isotope ratio mass spectrometry

Secondary outcomes

  1. L[13C]phenylalanine Oxidation

    Time frame: 7-weeks

    Measured by gas chromatography-mass spectrometry

Study contacts

Contact information is provided by the study sponsor or research team.

Damian Papadopoulos, B.Sc

CONTACT

[email protected]

(514) 561-7860

Tyler A. Churchward-Venne, Ph.D.

CONTACT

[email protected]

(514) 398-4184 ext. 00839

Sponsors and collaborators

Lead sponsor

McGill University

Other

Registry information

Important dates

Study start
2024
Primary completion
2026
Study completion
2026
First posted
May 28, 2024
Registry last updated
Feb 9, 2026

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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