Metabolic Unit
Lausanne, Canton of Vaud, 1000, Switzerland
NCT Number: NCT02041143
This trial is an exploratory study in order to determine whether the ingestion of one type of protein (milk protein/CHO/Fat) vs.placebo (CHO/Fat) 2 hours before bed time results in the extension of the post-prandial window of anabolism during the night by increasing whole body protein balance as well as muscle protein fractional synthesis rate (FSR).
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Notify Me55 year–65 year
Male
Interventional
Phase 1
Lausanne, Canton of Vaud, 1000, Switzerland
Healthy volunteers accepted: Yes
Only the study team can determine whether someone qualifies for participation.
Inclusion criteria
Exclusion criteria
Study product will provide 25 g of protein to subjects.
Product with no protein
Time frame: Within 24 hours of product intake
Muscle protein FSR (fractional synthesis rate) will be determined using primed constant infusions of [13C6] phenylalanine for 5 hours starting around from 03:00 a.m. (see flow chart attached). Whole body protein balance will be measured throughout the night using [15N]glycine (200mg) which will be orally taken with the trial product in the evening.
Time frame: Within 24 hours after product intake
Activation of signaling pathways involved in skeletal muscle protein balance will be assessed through use of immunohistochemistry
Time frame: Within 24 hours after product intake
Glucose metabolism will be assessed from circulating concentrations of glucose and insulin.
Time frame: Within 24 hours after product intake
The quantification of Free Amino Acids in Human Plasma will be done by Amino Acid Analyser Biochrome 30.
Société des Produits Nestlé (SPN)
Industry
Effects of Protein Intake on Muscle Protein Synthesis and Whole Body Protein Balance During an Overnight Fast: an Exploratory Study
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