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NCT Number: NCT07459465

Postprandial Triglyceride Concentrations Across Menstrual Cycle Phases

The goal of this clinical trial is to investigate the effect of menstrual cycle phases on postprandial triglyceride concentrations in healthy young female adults. The main question it aims to answer is: do postprandial triglyceride concentrations differ between the follicular and luteal phases of the menstrual cycle?

Participants will: visit the laboratory for a preliminary screening session to assess eligibility, and undergo two experimental sessions consisting of six hours of seated rest following the consumption of a high-fat meal (one session conducted in the early follicular phase, and one session conducted in the mid-luteal phase of the menstrual cycle).

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Key information

Age range

18 year–30 year

Sex eligibility

Female

Study type

Interventional

Phase

Not applicable

Primary location

Behavioural and Metabolic Research Unit, School of Human Kinetics, Faculty of Health Sciences, University of Ottawa

Ottawa, Ontario, K1S 5S9, Canada

Location status: Recruiting

Location contact

Pascal Imbeault

CONTACT

[email protected]

16135625800 ext. 7290

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • English or French speaking
  • Ability to provide informed consent

Exclusion criteria

  • History or evidence of chronic disease
  • Current use of hypolipemic medication
  • Current use of hormonal contraceptives
  • Current use of antidepressants
  • Current use of anticoagulants
  • Ongoing smoking status
  • Experiencing pregnancy, puerperium, or irregular menstrual cycles

Treatment and study plan

Early Follicular Phase

Other

In the early follicular phase of the menstrual cycle, participants will undergo 6 hours of seated rest after the consumption of a high-fat meal representing 33% of estimated daily energy expenditure and consisting of 59% of calories from fat.

Mid-Luteal Phase

Other

In the mid-luteal phase of the menstrual cycle, participants will undergo 6 hours of seated rest after the consumption of a high-fat meal representing 33% of estimated daily energy expenditure and consisting of 59% of calories from fat.

Primary outcomes

  1. Change from baseline in plasma total triglyceride concentrations

    Time frame: 6 hours

    Plasma total triglyceride concentrations will be measured via colorimetric assays from venous blood samples collected upon arrival at the laboratory (baseline) and during the exposure (i.e., 0 or baseline, 30, 60, 90,120, 180, 240, 300, and 360 minutes).

  2. Change from baseline in plasma buoyant triglyceride concentrations

    Time frame: 6 hours

    Plasma buoyant triglyceride concentrations will be measured via colorimetric assays from venous blood samples collected upon arrival at the laboratory (baseline) and during the exposure (i.e., 0 or baseline, 30, 60, 90,120, 180, 240, 300, and 360 minutes).

  3. Change from baseline in plasma denser triglyceride concentrations

    Time frame: 6 hours

    Plasma denser triglyceride concentrations will be measured via colorimetric assays from venous blood samples collected upon arrival at the laboratory (baseline) and during the exposure (i.e., 0 or baseline, 30, 60, 90,120, 180, 240, 300, and 360 minutes).

  4. Change from baseline in plasma non-esterified fatty acid concentrations

    Time frame: 6 hours

    Plasma non-esterified fatty acid concentrations will be measured via colorimetric assays from venous blood samples collected upon arrival at the laboratory (baseline) and during the exposure (i.e., 0 or baseline, 30, 60, 90,120, 180, 240, 300, and 360 minutes).

  5. Change from baseline in plasma insulin concentrations

    Time frame: 6 hours

    Plasma insulin concentrations will be measured via colorimetric assays from venous blood samples collected upon arrival at the laboratory (baseline) and during the exposure (i.e., 0 or baseline, 30, 60, 90,120, 180, 240, 300, and 360 minutes).

  6. Change from baseline in plasma glucose concentrations

    Time frame: 6 hours

    Plasma glucose concentrations will be measured via colorimetric assays from venous blood samples collected upon arrival at the laboratory (baseline) and during the exposure (i.e., 0 or baseline, 30, 60, 90,120, 180, 240, 300, and 360 minutes).

  7. Change from baseline in plasma β-hydroxybutyrate concentrations

    Time frame: 6 hours

    Plasma β-hydroxybutyrate concentrations will be measured via colorimetric assays from venous blood samples collected upon arrival at the laboratory (baseline) and during the exposure (i.e., 0 or baseline, 60, 120, 180, 240, 300, and 360 minutes).

Secondary outcomes

  1. Baseline serum β-estradiol concentrations

    Time frame: Baseline

    Serum β-estradiol concentrations will be measured via colorimetric assays from venous blood samples collected upon arrival at the laboratory (baseline)

  2. Baseline serum progesterone concentrations

    Time frame: Baseline

    Serum progesterone concentrations will be measured via colorimetric assays from venous blood samples collected upon arrival at the laboratory (baseline)

  3. Fasting resting energy expenditure

    Time frame: Baseline

    Fasting resting energy expenditure will be measured via indirect calorimetry upon arrival at the laboratory (baseline).

  4. Change from baseline in respiratory exchange ratio

    Time frame: 6 hours

    Respiratory exchange ratio will be measured via indirect calorimetry upon arrival at the laboratory (baseline), and hourly for 6 hours following meal consumption.

  5. Change from baseline in desire to eat

    Time frame: 6 hours

    Scores of subjective desire to eat, measured using visual analog scales. Measurements are performed upon arrival at the laboratory (baseline) and hourly during the 6-hour exposure. Scores will be measured using semantic differential scales in the form of 100-mm visual analog scales. For desire to eat, the question will be asked as follows: "How strong is your desire to eat?" (very weak (0 mm) - very strong (100 mm)).

  6. Change from baseline in hunger

    Time frame: 6 hours

    Scores of subjective hunger, measured using visual analog scales. Measurements are performed upon arrival at the laboratory (baseline) and hourly during the 6-hour exposure. Scores will be measured using semantic differential scales in the form of 100-mm visual analog scales. For hunger, the question will be asked as follows: "How hungry do you feel?" (not hungry at all (0 mm) - as hungry as I have ever felt (100 mm))

  7. Change from baseline in fullness

    Time frame: 6 hours

    Scores of subjective fullness, measured using visual analog scales. Measurements are performed upon arrival at the laboratory (baseline) and hourly during the 6-hour exposure. Scores will be measured using semantic differential scales in the form of 100-mm visual analog scales. For fullness, the question will be asked as follows: How full do you feel?" (not full at all (0 mm) - very full (100 mm)).

  8. Change from baseline in prospective food consumption

    Time frame: 6 hours

    Scores of subjective prospective food consumption, measured using visual analog scales. Measurements are performed upon arrival at the laboratory (baseline) and hourly during the 6-hour exposure. Scores will be measured using semantic differential scales in the form of 100-mm visual analog scales. For prospective food consumption, the question will be asked as follows: "How much food do you think you could eat?" (nothing at all (0 mm) - a large amount (100 mm)).

  9. Change from baseline in resting energy expenditure

    Time frame: 6 hours

    Resting energy expenditure will be measured via indirect calorimetry upon arrival at the laboratory (baseline) and hourly for 6 hours following meal consumption. The thermic effect of food is defined as the increase in resting energy expenditure above the fasting baseline value.

  10. Change from baseline in carbohydrate oxidation rate

    Time frame: 6 hours

    Carbohydrate oxidation rate will be measured via indirect calorimetry upon arrival at the laboratory (baseline) and hourly for 6 hours following meal consumption.

  11. Change from baseline in lipid oxidation rate

    Time frame: 6 hours

    Carbohydrate oxidation rate will be measured via indirect calorimetry upon arrival at the laboratory (baseline) and hourly for 6 hours following meal consumption.

Study contacts

Contact information is provided by the study sponsor or research team.

Pascal Imbeault, PhD

CONTACT

[email protected]

6135625800 ext 7290

Sponsors and collaborators

Lead sponsor

University of Ottawa

Other

Collaborators

  • Hopital Montfort
  • Institut du Savoir Montfort
  • Natural Sciences and Engineering Research Council, Canada

Registry information

Official study title

Effect of Menstrual Cycle Phases on Postprandial Triglyceride Concentrations in Healthy Premenopausal Females: A Crossover Study

Important dates

Study start
2026
Primary completion
2026
Study completion
2026
First posted
Mar 9, 2026
Registry last updated
May 19, 2026

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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