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OpenTrials
Completed

NCT Number: NCT01319513

Post-exercise Dietary Protein Strategies

Protein ingestion increases the rate at which the body builds new proteins in skeletal muscle (muscle protein synthesis. This study is designed to examine how the pattern of feeding affects muscle protein synthesis following resistance exercise. There is reason to believe that the large rapid increase in blood amino acid concentrations that accompanies the ingestion of a bolus of protein is important to increasing muscle protein synthesis. Thus, we hypothesize that the consumption a bolus of protein will elevate muscle protein synthesis to a greater extent than the consumption of an equivalent amount of protein that is consumed in small divided doses.

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Key information

Conditions

Optimal Anabolic Nutrition Interventions

Age range

18 year–35 year

Sex eligibility

Male

Study type

Interventional

Phase

Not applicable

Primary location

Ivor Wynne Centre A103, McMaster University

Hamilton, Ontario, L8S 4K1, Canada

About this study

The rapid appearance into the blood of essential amino acids, and leucine in particular, may act as an important signal to stimulate muscle protein synthesis after resistance exercise. This may explain why consuming rapidly-absorbed whey protein may have an anabolic edge over slowly-absorbed proteins such as casein. Previous investigations into importance of the rate of absorption to muscle protein synthesis that have used 'fast' and 'slow' proteins have been confounded by differences in amino acid composition. The present study addresses this issue by administering the same protein source, whey, as either a bolus or in small divided 'pulse' doses to achieve divergent amino acid profiles after a bout of resistance exercise.

This study is being conducted in young (18-35) men.

Our outcome measures include: blood amino acid concentrations, rates of myofibrillar protein synthesis, anabolic intracellular signalling markers

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • Non-obese men (BMI <27) between the age of 18 and 35 yrs.

Exclusion criteria

  • Type II diabetes or other known diseases
  • Use of medication
  • Female
  • Other ages or BMI than indicated above
  • Resistance training > 3X/wk

Treatment and study plan

whey protein bolus

Other

single dose of 25 g whey protein

whey protein pulses

Other

10 2.5 g pulses of whey protein

Primary outcomes

  1. Rate of myofibrillar protein synthesis

    Time frame: 4 months

    Rates of myofibrillar protein synthesis will be measured from muscle biopsy samples obtained from subjects participating in the study protocol.

Secondary outcomes

  1. Myocellular protein phosphorylation

    Time frame: 4 months

    Protein phosphorylation of target proteins will be measured from Western blot analysis of muscle biopsy samples.

Sponsors and collaborators

Lead sponsor

McMaster University

Other

Collaborators

  • Australian Institute of Sport
  • Nestec Ltd.
  • RMIT University

Registry information

Official study title

The Effect of the Pattern Post-exercise Aminoacidemia on Myofibrillar Protein Synthesis

Important dates

Study start
2009
Primary completion
2009
Study completion
2009
First posted
Mar 21, 2011
Registry last updated
Mar 21, 2011

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.