Polyphenols, Prebiotics, the Gut Microbiome and Stress

• All participants will be asked to fill out a health screening questionnaire and inclusion/exclusion criteria will be reviewed for volunteer eligibility
• Valid informed consent will be obtained from the volunteer.
• Once the study begins, participants will be randomly allocated into 1 of 4 groups each assigned a different polyphenol or prebiotic product. the prebiotic content is dosed as follows: Inulin at 5g/d, GOS at 5g/d and Resistant Starch at 20g/d.
• Participants will be asked to consume their assigned product once per day. The length of the intervention is 14 days
• Participants will provide two stool samples: one on day 0 and one on day 14 of the study to identify changes in gut microbiota composition
• Urine samples will also be provided at day 0 and at day 14 looking for changes in urinary metabolites
• Participants will also be asked to fill out various mood related questionnaires on days 0 and 14 of the study.
• You will take your own blood pressure and heart rate on day 0 and day 14 and at least 3 times per week to try and link changes in gut microbiota composition with vascular health.
• Volunteers will be given containers and specimen pots to take home for initial and final faecal and urine collections. No treatment will be issued until initial stool and urine sample has been provided
• Maintenance of normal dietary patterns throughout the study is essential and participants will be required to complete food and drink logs throughout the study via a web-based app - on three consecutive days with one of the days being a weekend

Inclusion criteria

• Volunteer is healthy at the time of pre-examination
• Volunteer has high perceived stress levels (own self-assessment)
• Volunteer is aged ≥ 18 to ≤ 65 years at the time of pre-examination
• Volunteer is able and willing to comply with the study instructions
• Volunteer is suitable for participation in the study according to the investigator/study personnel
• Written informed consent is given by volunteer

Exclusion criteria

• No command of any local language
• Gastrointestinal disorders including IBS, IBD or other conditions that might affect the gut environment
• Food allergies or intolerances
• Using drugs (e.g. antibiotics) influencing gastrointestinal function (8 weeks before intervention)
• Use of laxatives
• Participants with any form of diagnosed diabetes (types I and II)
• Volunteers currently involved or will be involved in another clinical or food study
• History of drug (pharmaceutical or recreational) or alcohol abuse.
• participants are pregnant or are lactating
• Regular intake of probiotic or prebiotic supplements
• Smoking