Pilot Trial of Fisetin in Healthy Volunteers and Older Patients With Multimorbidity

The goal of this pilot trial is to conduct a controlled clinical study to gather data on the pharmacokinetic profile of fisetin and its metabolites and on the safety and tolerability of fisetin in healthy volunteers as well as in older medical patients. Furthermore, we aim to identify potential outcome measures and perform sample size calculations for these outcomes, with the intent to conduct a larger scale effect study, at later date, given the result from this pilot study suggests that this would be feasible and safe.

The trial consists of:

• a single-arm open-label study, in which healthy volunteers (n=20) will receive fisetin corresponding to 20 mg/kg/day for two consecutive days.
• a 2-arm triple-blind randomized placebo-controlled study, in which older medical patients (n=40) will receive either:

• 20 mg/kg/day fisetin for two consecutive days, or
• placebo for two consecutive days.

Each of the studies (open-label study and randomized placebo-controlled study) consists of three sub-studies:

• Sub-study I aims to investigate the pharmacokinetic properties of fisetin and its main metabolites following oral administration at a dose of 20 mg/kg/day in healthy volunteers and in older medical patients.
• Sub-study II aims to assess the safety and tolerability of oral treatment with fisetin at a dose of 20 mg/kg/day fisetin for two consecutive days in healthy volunteers and in older medical patients.
• Sub-study III aims to gather representative measurements to assess the utility of inflammation, SASP, senescence, senolysis, and aging biomarkers, as well as measures of frailty, clinical parameters, physical and cognitive function, and quality of life as potential outcomes in future clinical trials; additionally, to perform sample size calculations for future trials based on these data.

Healthy volunteers:

Inclusion criteria

• Aged 20-35 years
• suPAR levels <3.5 ng/mL (± 15% corresponding to assay variation)
• Able to cooperate cognitively
• Able to read and understand Danish
• Women of childbearing potential must use effective contraception

Exclusion criteria

• Body weight >100 kg
• Inability to swallow pills
• Pregnant and/or lactating
• Known hypersensitivity or allergy to fisetin or excipients in the placebo capsules
• Presence of any condition that the investigator believes would put the subject at risk or would preclude the participant from successfully completing all aspects of the trial
• Presence of known chronic diagnosis
• Active acute illness
• Prescribed medication, except contraceptives
• Previous cancer diagnosis or treatment
• Use of senolytic and other "anti-aging" supplements

Older patients with multimorbidity:

Inclusion criteria

At screening #1 during hospital admission:

• Acutely hospitalized medical patient
• Age ≥65 years
• suPAR >5 ng/mL (± 15% corresponding to assay variation)
• Multimorbidity (≥2 chronic diagnoses)
• Able to cooperate cognitively
• Able to read and understand Danish

At screening #2 28 days after hospital discharge:

• suPAR >5 ng/mL (± 15% corresponding to assay variation)

Exclusion criteria

At screening #1 during hospital admission:

• Body weight >100 kg
• Inability to swallow pills
• Known human immunodeficiency virus infection, active hepatitis B or C infection, invasive fungal infection
• Uncontrolled (as per clinical judgment) pleural/pericardial effusions or ascites
• New/active invasive cancer except non-melanoma skin cancers
• Active cancer treatment or disseminated cancer
• Known condition associated with major immunodeficiency
• Known hypersensitivity or allergy to fisetin or excipients in the placebo capsules
• Use of senolytic and other "anti-aging" supplements

At screening #2 28 days after hospital discharge:

• Body weight >100 kg
• CRP >30 mg/L (± 15% corresponding to assay variation)
• Inability to swallow pills
• Presence of any condition, or abnormal routine biochemistry test, that the investigator believes would put the subject at risk or would preclude the patient from successfully completing all aspects of the trial
• Unstable (as per clinical judgment) major disorders, e.g., cardiovascular, renal, endocrine, immunological, hepatic disorder, or cancer
• Estimated glomerular filtration rate (eGFR) <15 ml/min/1.73 m2 or as per clinical judgment (e.g., risk of acute kidney injury)
• Human immunodeficiency virus infection, known active hepatitis B or C infection, invasive fungal infection
• Uncontrolled (as per clinical judgment) pleural/pericardial effusions or ascites
• New/active invasive cancer except non-melanoma skin cancers
• Active cancer treatment or disseminated cancer
• Known condition associated with major immunodeficiency
• Known hypersensitivity or allergy to fisetin or excipients in the placebo capsules
• Subjects taking strong inhibitors or inducers of CYP3A4 or as per clinical judgment
• Subjects taking specified substrates with a narrow therapeutic range for CYP3A4 or as per clinical judgment
• Subjects taking specified inhibitors, inducers, or substrates of CYP2D6, CYP2C9, or CYP2C8, or as per clinical judgment
• Subjects regularly using drug classes or specific medications or as per clinical judgment
• Use of senolytic and other "anti-aging" supplements