Phenotypic and Functional Study of 4BL B Cells in Multiple Sclerosis (MS)

the aim to compare the proportion of peripheral (blood) 4 BL cells but also 4-BL cells in cerebro spinal fluid (CSF) in MS compared to healthy controls and to other inflammatory neurological disease but also non inflammatory neurological disease.

For all groups of patients and controls it will collect blood and CSF only once (at diagnosis time for patients).

Blood collect from healthy controls will come from transfusion volunteers and we won't have CSF from them.

For patients from the MS group, the blood collect will be sequential at diagnosis, 3, 6, 12 and 24 months after during the follow up.

In the blood and CSF we will evaluate:

• percentage of 4 BL cells. 4 BL cells are found using cytometric parameters
• capacity of 4 BL cells to induce inflammatory response in vitro: percentage of induced activated TCD8 proliferation after cell culture using extracellular and intracellular cytometric parameters

Inclusion criteria

for MS group:

• MS defined by McDonald 2017 criteria with a disease duration of less than 1 year
• between 18 and 60 years old patients
• naïve of any immune therapy or steroid intake
• patients who signed consent to the study

Inclusion criteria

for controls with inflammatory of non inflammatory neurological disease:

• patients who signed consent to the study
• between 18 and 60 years old patients
• naïve of any steroid intake or immune therapy

Inclusion criteria

for healthy controls:

• control who signed consent at transfusion center for their blood collect to be used for study
• between 18 and 60 years old patients
• naïve of any steroid intake or immune therapy

Exclusion criteria

• pregnancy or breast-feeding
• patients or controls unable to sign the consent or to consent