Oxidative DNA Damage Enhancement by Vitamin C Among Narghile Smokers and Non-smokers

This study aims to evaluate oxidative DNA damage among hookah smokers compared to non-smokers by measuring biomarkers of oxidative stress in blood samples. The objective is to determine the impact of hookah smoking on DNA integrity and overall oxidative status in adults.

Completed

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Key information

Conditions

Narghile Behavior Pipe Smoking Smoking Water Pipe Smoking

Age range

18 month–30 month

Sex eligibility

All sexes

Study type

Interventional

Phase

Not applicable

Primary location

University of Duhok

Duhok, semel, Iraq

About this study

Hookah smoking has become increasingly popular in many regions, yet its potential impact on oxidative stress and DNA integrity is not fully understood. This study aims to investigate the level of oxidative DNA damage among hookah smokers compared to non-smokers by analyzing biomarkers in blood samples. A case-control observational design will be used, involving two groups of adult participants: regular hookah smokers and age-matched non-smokers. Blood samples will be collected from all participants and analyzed for markers of oxidative stress, including 8-hydroxy-2'-deoxyguanosine (8-OHdG) and malondialdehyde (MDA), using validated biochemical methods. The findings are expected to provide insights into the biological consequences of hookah smoking and support the development of public health interventions.

Who can participate

Healthy volunteers accepted: No

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

Adults aged 18-30 years

Current narghile (hookah) smokers for at least 1 year

Willing to provide informed consent

Exclusion criteria

Healthy participant

Treatment and study plan

Vitamin C (Ascorbic Acid) regularly has been taken

Dietary Supplement

Primary outcomes

Oxidative DNA damage

Time frame: Baseline and after 1 month

Measurement of plasma MDA concentration using the thiobarbituric acid reactive substances (TBARS) method to assess oxidative stress in participants before and after 1-month Vitamin C supplementation and measuring oxidative stress marker and TAOC

Sponsors and collaborators

Lead sponsor

Nora Tariq Mohammed

Other

Registry information

Acronym: DNA Damage

Important dates

Study start: 2024
Primary completion: 2024
Study completion: 2025
First posted: Aug 26, 2025
Registry last updated: Aug 26, 2025

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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Completed