Oocyte Vitrification for In Vitro Fertilization (IVF) Patients and Women With Fertility Threatening Conditions

Inclusion criteria

• Women between the ages of 18 and 37 and who require IVF for a successful pregnancy will be approached to consent for the study,. They must also have a cycle day 3 FSH < 10 mIU/ml, estradiol < 70 pg/ml and a baseline follicle count > 10. The BMI must be between 20 and 32kg/m2. If they do consent, eggs will only be vitrified if 13 or more mature metaphase II oocytes are collected.
• Women between the ages of 14 and 42 who are seeking treatment for fertility preservation. In addition they must also meet the following criteria.
• Patient is willing and able to delay any relevant therapy for up to 40 days.
• Have functioning ovaries as determined by blood / ultrasound testing.
• Patient is willing to undergo infectious disease testing for HIV, hepatitis B and C, syphilis, gonorrhea and Chlamydia.
• The patient must have clearance from their oncologist or medical specialist, if any, to undergo ovarian stimulation and transvaginal oocyte retrieval.
• The patient must not have a past history of blood clots in the veins or in the lungs.
• The patient must be willing to undergo transvaginal, internal ultrasound examination and pelvic examination.

Exclusion criteria

• Patients with a cycle day 3 FSH > 10 mIU/ml, cycle day 3 estradiol > 70 pg/ml and a baseline follicle count < 10 will be excluded.
• Patients with a BMI <20 and >32 will be excluded.
• Patients who are not willing or are unable to meet the above mentioned eligibility requirments.
• Patients with expected low ovarian reserve [evaluated on day 3 by an antral follicle count of <3 follicles at 2-5 mm diameter and/or an increased concentration of follicle stimulating hormone (FSH) >15 IU [15] on cycle day 3 ].
• Patients with possible poor quality of oocytes, based on medical history including previous exposure to chemotherapeutics or radiation.