Nonmyeloablative Stem Cell Transplantation With CD8-depleted or Unmanipulated Peripheral Blood Stem Cells (PBSC)

• Patients

1.1. Diseases

Malignant diseases confirmed histologically and not rapidly progressing:

• Hematologic malignancies
• AML;
• ALL;
• CML and other myeloproliferative disorders;
• MDS;
• Multiple myeloma;
• CLL;
• Non-Hodgkin's lymphoma;
• Hodgkin's disease.
• Non-hematologic malignancies
• Renal cell carcinoma (metastatic).

1.2. Inclusion criteria

• Male or female; female patients must use a reliable contraception method;
• Age lower than 70 yrs (family donor) or lower than 65 yrs (unrelated donor);
• HIV negative;
• No terminal organ failure;
• No uncontrolled infection, arrhythmia or hypertension;
• Family donor (HLA-identical) or unrelated donor (matched for A-B by low resolution typing and for DRB1-DQB1 by high resolution typing);
• No previous radiation therapy precluding the use of 2 Gy TBI
• Informed consent given by patient or his/her guardian if of minor age.

1.3. Clinical situations

• Theoretical disease indication for a standard allo-transplant, but not feasible because:
• Age > 55 yrs;
• Unacceptable end organ performance;
• Patient's refusal.
• Indication for a standard auto-transplant:

• perform mini-allotransplantation 2-6 months after standard autotransplant.
• Not an indication for intensification but a potential candidate for cellular immunotherapy.
• Donors

2.1. Inclusion criteria

• Related to the recipient (sibling, parent or child) or unrelated;
• Male or female;
• Weight > 15 Kg (because of leukapheresis);
• HIV negative;
• No major contraindication for allogeneic PBSC donation by generally accepted criteria;
• Informed consent given by donor or his/her guardian if of minor age.

2.2. Exclusion criteria

• Any condition not fulfilling inclusion criteria;
• Unable to undergo leukapheresis because of poor vein access or other reasons.