Skip to main content
OpenTrials
Recruiting

NCT Number: NCT05372861

Nanopore Sequencing in Ophthalmology

In this pilot study, the feasibility of the Oxford Nanopore MinION to identify pathogens from vitrectomy samples in eyes with endophthalmitis shall be assessed. The MinION is a low cost commercially available device for DNA/ RNA analysis that, in studies, has been used for pathogen determination in various infectious diseases as well as for the genetic characterization of hematologic tumors.

Recruiting

Interested in participating?

Request Info

Key information

Conditions

Endophthalmitis Eye Diseases Eye Infections Infections

Age range

18 year–99 year

Sex eligibility

All sexes

Study type

Observational

Primary location

About this study

Vitreous samples and anterior chamber taps from eyes with endophthalmitis are taken during routine vitrectomy. The gathered samples are divided in half to undergo routine culture for microbial growth and nanopore sequencing with the MinION Mk1b, a commercially available device for DNA/RNA sequencing. Base reads from Nanopore sequencing are then compared with available libraries for pathogen identification. The Nanopore sequencing results for pathogens are compared versus the results of the microbiological culture of the same eye as the gold standard. Clinical features and visual function before and after vitrectomy are gathered from patient charts. Treatment is not altered due to results of the Nanopore sequencing.

Who can participate

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • Eyes of patients with endophthalmitis

Exclusion criteria

  • No informed consent possible
  • Participation in other studies

Treatment and study plan

Primary outcomes

  1. Pathogen detection

    Time frame: In vitro analysis after vitrectomy for endophthalmitis. Start of analysis: Within the next working day after sample acquisition. Time for Nanopore sequencing: Approximately up to 10 hours. Time for culture: 24 hours up to 14 days.

    Pathogens detected by Nanopore sequencing versus microbiologic culture

Secondary outcomes

  1. Time to pathogen detection

    Time frame: Time measured from start of analysis until arrival of results. Time for Nanopore sequencing: Approximately up to 10 hours. Time for culture: 24 hours up to 14 days.

    Time in days from gathering of the sample until arrival of results

  2. Visual acuity

    Time frame: Gathered from patient charts two months after vitrectomy.

    Visual acuity measured with Snellen charts

Study contacts

Contact information is provided by the study sponsor or research team.

Gerald Seidel, MD, PhD

CONTACT

[email protected]

004331638580714

Michael Sommer, MD

CONTACT

[email protected]

004331638531033

Sponsors and collaborators

Lead sponsor

Medical University of Graz

Other

Registry information

Official study title

Nanopore Sequencing in Ophthalmology - a Paradigm Shift in Pathogen Determination?

Important dates

Study start
2022
Primary completion
2026
Study completion
2027
First posted
May 13, 2022
Registry last updated
Sep 30, 2025

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

Related clinical trials

Published trials that share one or more normalized conditions with this study.