Molecular Evaluation of Neoadjuvant Chemotherapy for Locally Advanced Gastric Cancer

This study is a single-center observational study on a patient cohort of at least 80 patients with histologically-confirmed locally advanced gastric cancer (LAGC). The protocol used in this study is approved by the Ethics Committee of Beijing Cancer Hospital.

The primary endpoint is the 3-year progression-free survival (PFS) rate. The secondary endpoints are the overall survival (OS) and safety.

Currently, the best treatment for early and mid-stage LAGC patients is resection but even with successful treatment, most patients still relapse and the 5-year survival rate is less than 30%.

For patients with cT4a/T4bN+M0, including T4b、Bulky-N2, primary lesions are not always fully excised during treatment and prognosis for these patients is generally poor. Recent studies, however, have suggested that the inclusion of neoadjuvant chemotherapy (NCT) can improve patient outcomes by: 1) downstaging tumors and increasing the likelihood of curative resection, 2) reducing the prevalence of micro metastases.

Historically, Oxaliplatin and s-1 combination therapy has been shown to be well-tolerated in patients with recurrent or metastatic gastric cancer.

To best evaluate the treatment response of NCT, we plan to investigate the effect of new technologies and assays on the successful prediction of patient outcomes.

Circulating tumor DNA (ctDNA), fragmented DNA with an average size of 166 bp, is released by cancer cells into circulation. Circulating tumor cells (CTCs) are rare malignant cells detached from tumors which enter the bloodstream. Both these biomarkers can be used for prognosis and the dynamic monitoring of disease progression.

In the MAGIC trial, patients with tumor that are MSI-H or MMRD, had survival rates superior to those with MSS/MSI-L or MMRP tumors when treated with surgery alone.

We will combine dMMR/MSI status with the dynamic evaluation of CTCs and ctDNA using liquid biopsy technology to determine whether changes in tumor burden in response to NTC can identify potential treatment responders.

Sequential peripheral blood samples for CTCs and ctDNA analysis will be taken before and after NCT, as well as one week after surgery.

Tumor assessments will be performed after 2 cycles NCT based on RECIST v1.1 criteria using CT/MRI scan.

Inclusion criteria

• Ambulatory males or females, age ≥ 18 years
• Karnofsky Performance Score (KPS) ≥70% or ECOG performance status: 0 or 1
• Pathologically confirmed gastric adenocarcinoma (regardless of degree of histologic differentiation) or adenocarcinoma with signet-ring cell carcinoma , mucinous adenocarcinoma
• Clinical Preoperative Stage cT4a/T4bN+M0 disease, including T4b、Bulky-N2, confirmed by CT/EUS
• Adequate organ function as defined below: Hemoglobin ≥ 9 g/dl, Hematologic Absolute Neutrophil Count （ANC） ≥ 1.5*109/L, Platelets ≥ 100*109/L, Aspartate Aminotransferase（AST） and Alanine Aminotransferase(ALT)≤ 2.5×ULN, Alkaline pPosphatase( ALP) ≤ 2.5×ULN, Total Bilirubin （TBIL）≤ 1.5×ULN, Renal Serum Creatinine < 1.5 ULN, Serum Albumin ≥ 30g/l.
• No serious concomitant disease that make survival period < 5 years
• No pleural effusion, no ascites exceeding the pelvis and no metastasis to the peritoneum, liver or other distant organs are confirmed by abdominal pelvic CT.
• Planning to undergo gastric cancer D2 surgery after neoadjuvant chemotherapy
• No prior antitumor treatment is allowed, including chemotherapy, radiotherapy, immune therapy or target therapy
• No mechanical obstruction.
• Negative serum or urine pregnant test within 7 days prior to randomization for child-bearing age women
• Sexually active males or females willing to practice contraception during the study until 30 days after end of study.
• Subjects has to voluntarily join the study and sign the Informed Consent Form for the study

Exclusion criteria

• Female in pregnancy or lactation, or refuse to receive Contraception measures during chemotherapy
• With distant metastasis or peritoneal dissemination diagnosed by CT/EUS
• Underwent prior antitumor treatment, including chemotherapy, radiotherapy, immune therapy or target therapy
• Serious uncontrolled intercurrent infections or other serious uncontrolled concomitant disease or condition that would make the subject inappropriate for study participation or any serious medical disorder that would interfere with the subject's safety (including current active hepatic, biliary, renal, respiratory disease, uncontrolled diabetes hypertension et al)
• Clinically serious cardiac disease or pulmonary dysfunction.
• Patients require emergency surgery with complications (bleeding, perforation and obstruction) caused by gastric cancer
• Other complications that cause no radical resection
• Serious concomitant disease that make survival period < 5 years
• No detection of CTCs or ctDNA in peripheral blood samples before NCT be enrolled in other clinical trials
• Allergic reaction to S-1 or oxaliplatin
• Abnormal GI tract function
• Refuse to provide blood/tissue sample
• Sexually active males or females refuse to practice contraception during the study until 30 days after end of study.
• Person with no capacity (legally) or inappropriate to continue study treatment for ethics/medical reasons.
• Other situation to be judged not adaptive to the study by investigators