MMP-2/3 and Periapical Healing Outcomes

This prospective randomized clinical study will investigate the association between MMP-2 and MMP-3 gene polymorphisms and radiographic healing outcomes following nonsurgical root canal treatment in patients with chronic apical periodontitis. A total of 50 patients will undergo standardized endodontic treatment and will be followed for 12 months. Genotyping will be performed using baseline DNA samples, and periapical healing will be assessed using the Periapical Index (PAI). The relationship between genetic variations and treatment outcomes will be analyzed to determine whether host-related genetic factors influence healing patterns after root canal therapy.

Not Yet Recruiting

Trial opening soon.

Get Notified

Key information

Conditions

Chronic Apical Periodontitis Periapical Healing

Age range

18 year–65 year

Sex eligibility

All sexes

Study type

Observational

Who can participate

Healthy volunteers accepted: No

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

Single-rooted teeth
PAI score ≥ 3
Fully developed root apices
Periodontal probing depth less than 5 mm
Indication for primary nonsurgical root canal treatment

Exclusion criteria

Previous endodontic treatment in the selected tooth
Diagnosis of symptomatic apical abscess
Presence of root fracture or internal/external resorption
Use of systemic antibiotics within the previous 4 weeks
Analgesic intake within 48 hours before treatment
Ongoing immunosuppressive therapy
Pregnancy or breastfeeding

Treatment and study plan

Nonsurgical Root Canal Treatment

Procedure

All participants will undergo standardized nonsurgical root canal treatment performed under a uniform clinical protocol. After local anesthesia and rubber dam isolation, working length will be determined electronically and radiographically. Root canal preparation will be completed with rotary instrumentation up to size 30/.06, accompanied by sodium hypochlorite irrigation, EDTA, and ultrasonic activation. Canals will then be obturated in the same visit using gutta-percha and epoxy resin-based sealer with the warm vertical condensation technique. Periapical healing outcomes at 12 months will be evaluated in relation to MMP-2 and MMP-3 genetic polymorphisms.

Primary outcomes

Radiographic Periapical Healing

Time frame: 12 months after treatment

The primary outcome will be radiographic healing assessed using the Periapical Index (PAI) scoring system on standardized periapical radiographs.

Secondary outcomes

Association Between MMP Genotypes and Healing Outcomes

Time frame: Baseline (genotyping) and 12 months after treatment

The association between MMP-2 (rs243865) and MMP-3 (rs3025058) genotypes and radiographic healing outcomes will be evaluated. Genotype and allele distributions will be compared between healed and non-healed groups to determine the potential influence of genetic polymorphisms on periapical healing following root canal treatment.

Sponsors and collaborators

Lead sponsor

TC Erciyes University

Other

Registry information

Official study title

Association of MMP-2 and MMP-3 Polymorphisms With 12-Month Radiographic Healing After Nonsurgical Root Canal Treatment in Chronic Apical Periodontitis: A Prospective Clinical Study

Important dates

Study start: 2026
Primary completion: 2027
Study completion: 2027
First posted: Apr 14, 2026
Registry last updated: Apr 14, 2026

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

Published trials that share one or more normalized conditions with this study.

Not Yet Recruiting