Microdosing of BAC ONE to the Distal Lung

The primary objective of this study is to deliver a BAC ONE microdose to ventilated controls and patients with lung infection to assess the imaging parameters of BAC ONE over human autofluorescence and to assess if bacteria can be detected in vivo in situ within the distal lung.

The primary endpoint is to visualise the delivery of a microdose of BAC ONE and assess imaging parameters in:

• 3 mechanically ventilated patients to provide a control population (cohort 1)
• 6 bronchiectasis patients with predominant colonisation with gram-negative bacteria (cohort 2)
• 6 bronchiectasis patients with predominant colonisation with gram-positive bacteria (cohort 3)
• 3 patients with suspected pneumonia and pulmonary infiltrates in ICU (cohort 4)

For all cohorts, eligibility will be verified by a clinical trial physician after written informed consent has been obtained. For all cohorts, a bronchoscopy with lavage will be performed to harvest broncho-alveolar lavage fluid (BALF). Fibre-based endomicroscopy (FE) will be performed on up to three areas and up to 80μg (± 25%) in total of BAC ONE will be instilled in up to 3 sites.

Participants will be asked to provide additional blood and urine samples with the intention of examining for systemic uptake of the BAC ONE probe. Routine blood investigations will be performed 4-6 hours following the administration of BAC ONE. All participants will either be contacted by a member of the research team via telephone or ward visit 24 hours (± 4 hours) after BAC ONE dosing to ensure no AEs/SAEs were experienced. The participant's involvement in the study is concluded when the 24 hour assessment has been successfully completed and all AEs/SAEs have been resolved.

Inclusion criteria

All cohorts

• ≥ 16 years
• Attending consultant permission for bronchoscopy

Cohort 1

• Patients scheduled to undergo surgery under general anaesthesia
• Absence of acute or significant chronic lung disease as determined by the clinical suspicion of the attending medical team or of a medically qualified member of the study investigation team.
• Scheduled presence of an endo-tracheal tube (ETT).
• Capacity to provide informed consent

Cohort 2 and 3

• Patients with bronchiectasis with known microbiological predominance of gram-negative or gram-positive bacteria.
• Capacity to provide informed consent

Cohort 4

• Patients in the ICU with suspected VAP and pulmonary infiltrates on radiological assessment
• Presence of invasive tracheal ventilation tube
• Provision of informed consent from the patient or their personal legal representative prior to any study related procedures.

Exclusion criteria

All cohorts

• Refusal for participation by attending consultant
• Any history of anaphylaxis
• Significant coagulopathy, which causes bronchoscopy to be unsuitable, as determined by clinical co-investigator or the participant's attending consultant, using information which is routinely available
• Myocardial infarction in the preceding four weeks
• Women who are pregnant or are breastfeeding
• Receiving drugs that cause increased autofluorescence in the lung, specifically amiodorane and methotrexate

Participants in cohort 4 only

• Inspired Oxygen Concentration (FiO2) >70%
• Positive End Expiratory Pressure (PEEP) >10cm
• ETT or tracheostomy internal diameter < 7mm
• Presence of pneumothorax
• Active bronchospasm
• Mean arterial pressure <65mmHg AND on vasopressor
• Platelet count < 50 x 109/L