Microbiota and Protein-energy Wasting (MIDIWA)

General description :

This protocol is multicenter (University Hospitals of Geneva (HUG), Lausanne University Hospital (CHUV), Hospital of Sion (HVS), dialysis center of the Champel clinic in Geneva and dialysis center of the Cécil clinic in Lausanne) and encompasses two parts, a cross-sectional and a longitudinal study:

• Cross-sectional study: It is performed to differentiate the respective impact of uremia and protein-energy wasting (PEW) on gut barrier and gut microbiota. It will compare gut barrier of hemodialysis (HD) patients with PEW, with gut barrier of age-matched HD well-nourished patients, non dialyzed patients with chronic kidney disease (CKD), and healthy non obese volunteers (10 in each group). This study part is essential for interpretation of the changes occurring in the longitudinal study.
• Longitudinal double blind randomized crossover study: HD patients with PEW (36 patients), receive, in a randomized double-blind order, either BCAA or an isocaloric isonitrogenous placebo for 4 months each, with a wash-out period of 1 month.

Randomization :

• Cross-sectional study: No randomization will be performed. HD patients without PEW, non dialyzed patients with CKD and healthy non obese volunteers will be included if matched for gender and age with the included HD patients with PEW. Age-matching will allow a discrepancy of +/- 5 years compared to the HD patient.
• Longitudinal study: Sequence assignment will be randomized separately in each center to ensure an equal percentage of each sequence in both centers. For this purpose, the investigators will generate 2 lists of randomization with the method of randomly permuted blocks with random block sizes of 2 and 4.

Recruitment :

Patients will be recruited among the outpatients of the Nephrology Divisions or Services of the HUG, CHUV, HVS and Champel and Cécil clinics. Healthy volunteers will be recruited among hospital staff or their relatives.

Inclusion criteria

• HD patients with PEW

• Age ≥ 18 years.
• Maintenance HD for at least 3 months.
• Fasting predialysis plasma albumin < 38 g/l in the absence of an known acute infection during the last 2 weeks or body weight loss > 5% of estimated dry body weight over 3 months
• Dietary intakes (24h dietary recall) between 20-30 kcal/kg/d and < 1 g protein/kg/d on one occasion, during screening. These intakes will not include the intake of oral nutritional supplements, as intakes below 20 kcal/kg/d request artificial nutrition.
• Absence of any systematic antibiotic treatment for an acute infection in the month preceding the inclusion.
• HD patients without PEW

• Patients matched for age and gender to HD patients with PEW.
• Maintenance HD for at least 3 months.
• Fasting predialysis plasma albumin ≥ 40 g/l, in the absence of any known acute infection during the last 2 weeks
• Dietary intakes (24h dietary recall) > 30 kcal/kg/d and > 1.2 g protein/kg/d, once during screening.
• Absence of any systematic antibiotic treatment for an acute infection in the month preceding the inclusion.
• Non dialysed patients with chronic kidney disease stage 4

• Patients matched for age and gender to HD patients with PEW.
• Chronic kidney disease, stage 4 or 5, not requiring HD.
• Fasting predialysis plasma albumin ≥ 40 g/l, in the absence of any known acute infection during the last 2 weeks
• Dietary intakes (24h dietary recall) > 30 kcal/kg/d once during screening.
• Absence of any systematic antibiotic treatment for an acute infection in the month preceding the inclusion.
• Healthy non obese volunteers

• Subjects matched for age and gender to HD patients with PEW.
• Body mass index < 30 kg/m2
• Absence of chronic disease potentially leading to wasting or cachexia.
• Absence of plasma C-reactive protein > 50 mg/l in the last 2 weeks or known acute infection.
• Absence of any systematic antibiotic treatment for an acute infection in the month preceding the inclusion.

Inclusion criteria

• Age ≥ 18 years.
• Maintenance hemodialysis for at least 3 months.
• Fasting predialysis plasma albumin < 38 g/l in the absence of any known acute infection during the last 2 weeks or body weight loss > 5% of estimated dry body weight over 3 months
• Dietary intakes (24h dietary recall) between 20-30 kcal/kg/d and < 1 g protein/kg/d on one occasion, during screening. These intakes will not include the intake of oral nutritional supplements, as intakes below 20 kcal/kg/d request artificial nutrition
• Absence of any systematic antibiotic treatment for an acute infection in the month preceding the inclusion

Exclusion criteria

• Known psychiatric or cognitive disorder, precluding protocol compliance.
• Life expectancy below 1 year.
• Inadequate dialysis (Kt/V<1.2 on 3 consecutive occasions, for HD patients only), if applicable.
• Enteral or parenteral nutrition.
• Drugs or oral nutritional supplements containing fibers since ≤ 1 month.
• Known reasons for decreased plasma albumin levels as liver failure or exudative enteropathy.
• Drugs influencing body composition, ≤ 1 month : systemic corticosteroids, anabolic drugs as insulin or testosterone, post-menopausal hormone therapy, injectable contraceptives.
• Known endocrinological disorders potentially leading to hypo- or hypermetabolism, untreated or treated since ≤ 1 month : disorders of thyroid gland, adrenal glands...
• Pregnancy and breast-feeding.