Microbiological Effect of 0.12% Chlorhexidine Digluconate and 0.05% Cetylpyridinium Chloride vs Probiotics (Lactobacillus Reuteri Prodentis) on Porphyromonas Gingivalis.

Our study will be divided into two groups: in group 1, we will provide a mouthwash containing 0.12% chlorhexidine and 0.05% cetylpyridinium as treatment, and in group 2, patients will be administered oral probiotic capsules (Lactobacillus reuteri Prodentis). A periodontogram will be performed for both groups to determine the area of the mouth with the highest bacterial presence due to the deepest periodontal pocket. From there, a crevicular fluid sample will be taken from which the red complex bacteria causing periodontal disease will be obtained, using sterile #30 endodontic paper points.

Before the start of treatments, both groups will receive the mechanical Gold Standard treatment for periodontal disease, which is non-surgical periodontal therapy (dental prophylaxis). Both therapeutic agents will be administered for 4 weeks, after which a sample will be taken from the same periodontal pocket as the first one, and the microbiological effect of 0.12% chlorhexidine and 0.05% cetylpyridinium vs. probiotics (Lactobacillus reuteri Prodentis) will be evaluated. Patients will then have a rest period of 3 months, after which the treatments will be crossed, following the same previous procedure.

Inclusion criteria

• • Previous diagnosis of controlled type 2 diabetes mellitus.

• Periodontal disease (≥2 quadrants affected; probing depth ≥4 mm; bleeding on probing >10%).
• Patients affiliated with the Unidad de Salud Zona Oriente (IMSS-BIENESTAR).
• Signed informed consent.

Exclusion criteria

• Inability to cooperate.

• Antibiotic treatment within the last 3 months.
• Soft tissue pathologies not related to periodontal disease.

Elimination Criteria:

• Failure to complete the study.
• Insufficient or non-extracted DNA sample.
• Indication of antibiotics/fluoxetine during the intervention.