Completed

NCT Number: NCT05274334

Measurement of Lipogenic Flux by Deuterium Resolved Mass Spectrometry Via Non-invasive Breath Analysis

This proof of concept- study is to measure de novo lipogenesis (DNL) non-invasively in exhaled breath in overnight-fasted humans by triggering feeding response by deuterium resolved mass spectrometry.

Completed

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Key information

Conditions

De Novo Lipogenesis (DNL)

Age range

18 year and older

Sex eligibility

All sexes

Study type

Interventional

Phase

Not applicable

Primary location

University Children's Hospital Basel (UKBB)

Basel, 4056, Switzerland

About this study

The study objective is to measure the DNL by ingestion of a single oral dose of 70% 2H2O at 2 g/kg body-water. The subjects will consume a Yoghurt consisting of 300g of 'Excellence Joghurt Nature gezuckert' which contains 410 Kcal, 28g fat, 34g carbohydrates and 9g protein. The exhaled air of the subjects will be measured several times per hour to analyse de novo lipogenesis (DNL) non-invasive by deuterium resolved mass spectrometry.

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

Healthy adult volunteers
English speaking, or good knowledge of the English language
Able to understand the study
Able to give informed consent

Exclusion criteria

Current pregnancy

Treatment and study plan

Assessment of de novo lipogenesis non-invasively in exhaled breath

Other

Healthy participants have a good nights sleep whereas they don't eat during the night and until measurements.
They come to the lab and make first baseline exhalation sets. An exhalation set consists of 6 exhalations in Mass Spectrometer positive mode and the same in negative mode. One exhalation set takes around 7minutes from start to finish.
Participants consume a single oral dose of dose of 70% 2H2O at 2 g/kg body-water.
Participants execute several exhalation sets over time.
Participants consume 300g of 'Excellence Joghurt Nature gezuckert.
Participants execute several exhalation sets over time.
After around 4.5hours with regular exhalation sets taking place, the trial is concluded.

Primary outcomes

Variation of signal intensity of deuterated exhaled metabolites across the approximately lasting 4.5 hours trial

Time frame: approximately 4.5 hours

Variation of signal intensity of deuterated exhaled metabolites across the approximately lasting 4.5 hours trial

Sponsors and collaborators

Lead sponsor

University Children's Hospital Basel