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NCT Number: NCT07472010

Mandibular Stretching and 4-4-8 Breathing Exercises on Autonomic Nervous System Activity

Mandibular stretching exercises and breathing techniques may influence autonomic nervous system activity, muscle tone, and psychological state. This randomized controlled trial aims to investigate the effects of mandibular stretching exercise and the 4-4-8 breathing exercise on autonomic nervous system activity, muscle tone, and psychological parameters in healthy university students. Participants will be randomly assigned to intervention groups and evaluated before and after the intervention period.

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Key information

Age range

18 year–30 year

Sex eligibility

All sexes

Study type

Interventional

Phase

Not applicable

Primary location

Necmettin Erbakan University

Konya, Turkey (Türkiye)

Location status: Recruiting

About this study

The autonomic nervous system plays an important role in regulating physiological and psychological responses. Breathing techniques and mandibular stretching exercises are non-invasive approaches that may influence autonomic nervous system activity and muscle tone through neuromuscular and respiratory mechanisms. However, the combined or individual effects of these interventions on autonomic regulation and psychological outcomes remain insufficiently investigated.

This randomized controlled trial aims to examine the effects of mandibular stretching exercise and the 4-4-8 breathing exercise on autonomic nervous system activity, muscle tone, and psychological state in healthy university students. Participants will be randomly allocated to intervention groups according to the study protocol. Baseline and post-intervention measurements will be collected to evaluate changes in autonomic nervous system parameters, muscle tone, and psychological status.

The findings of this study may contribute to understanding the potential benefits of simple non-pharmacological interventions such as mandibular stretching and breathing exercises in improving physiological regulation and psychological well-being.

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • Inclusion Criteria:
  • Healthy university students
  • Age between 18 and 30 years
  • Voluntary participation in the study
  • Ability to understand and perform mandibular stretching exercise and 4-4-8 breathing exercise

Exclusion criteria

  • History of neurological disorders
  • Musculoskeletal disorders affecting the jaw, neck, or respiratory system
  • Respiratory diseases
  • Use of medications that may affect the autonomic nervous system
  • Inability to perform the exercises required in the study

Treatment and study plan

Mandibular Stretching Exercise

Other

Participants perform mandibular stretching exercises according to the study protocol

4-4-8 Breathing Exercise

Other

Participants perform the 4-4-8 breathing exercise according to the study protocol.

Primary outcomes

  1. Autonomic Nervous System Activity

    Time frame: Baseline and 20, 40, 80, and 120 minutes after the intervention

    Autonomic nervous system activity will be assessed by measuring heart rate (beats per minute) using a digital pulse oximeter and blood pressure (mmHg) using an automatic blood pressure monitor. Measurements will be recorded at baseline and repeatedly after the intervention period (20, 40, 80, and 120 minutes).

Secondary outcomes

  1. Muscle Tone

    Time frame: Baseline and 20, 40, 80, and 120 minutes after the intervention

    Muscle activity of the temporalis and masseter muscles will be assessed using surface electromyography (EMG). EMG amplitude (microvolts, µV) will be recorded at baseline and repeatedly after the intervention period (20, 40, 80, and 120 minutes).

  2. Psychological State

    Time frame: Baseline and 20, 40, 80, and 120 minutes after the intervention

    Psychological state will be assessed using the State-Trait Anxiety Inventory (STAI) questionnaire. STAI scores (range 20-80 points) will be recorded at baseline and repeatedly after the intervention period (20, 40, 80, and 120 minutes).

Study contacts

Contact information is provided by the study sponsor or research team.

Mehin Mammadzada, MSc Student

CONTACT

[email protected]

+905313124790

Sponsors and collaborators

Lead sponsor

Mehin Mammadzada

Other

Registry information

Official study title

Investigation of the Effects of Mandibular Stretching Exercise and 4-4-8 Breathing Exercise on Autonomic Nervous System Activity, Muscle Tone, and Psychological State: A Randomized Controlled Trial

Acronym: MSE-BR

Important dates

Study start
2025
Primary completion
2026
Study completion
2026
First posted
Mar 16, 2026
Registry last updated
Mar 16, 2026

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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