Skip to main content
OpenTrials
Completed

NCT Number: NCT04742244

Lemon Verbena Extract Oxidative Stress and Muscle Damage

In the present study, investigators investigated whether supplementation with lemon verbena extract (LVE) could improve muscle damage and biochemical indicators after exhaustive exercise challenge. Based on maximum jump heights, 60 subjects (30 males and 30 females) were equally divided into a placebo group (0 mg/human/day) and an LVE supplement group (400 mg/human/day), with gender-equal distribution. All subjects started supplementation 10 days before exhaustive exercise and continued it until all tests were completed. Before the intervention, after the exhaustive exercise, and on the following 3 days, the participants underwent 12-minute Cooper running/walking; blood collection; assessments of pain, muscle stiffness, maximum jump heights, and isometric maximum muscle strength; and anaerobic strength tests.

Completed

Looking for future studies?

Notify Me

Key information

Conditions

Muscle Injury Muscle Soreness Muscular Diseases Musculoskeletal Diseases Musculoskeletal Pain Myalgia Nervous System Diseases Neurologic Manifestations Neuromuscular Diseases Pain Pathological Conditions, Signs and Symptoms Signs and Symptoms

Age range

20 year–30 year

Sex eligibility

All sexes

Study type

Interventional

Phase

Not applicable

Primary location

National Taiwan Sport University

Taoyuan, 333, Taiwan

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • health
  • Must be able to swallow tablets

Exclusion criteria

  • non-smokers
  • no musculoskeletal
  • medical
  • metabolic disease
  • women not on the reproductive cycle
  • high blood pressure, asthma
  • skeletal neuromuscular injuries in the upper or lower extremities

Treatment and study plan

Lvemon verbena extract

Dietary Supplement

Each capsule contained 200 mg of lemon verbena extract 2 capsules/day, 14 days

Maltodextrin

Dietary Supplement

Each capsule contained 0 mg of lemon verbena extract 2 capsules/day, 14 days

Primary outcomes

  1. inflammation of IL-6 level

    Time frame: 10 days

    To assess inflammation indicators, volunteers fasted for at least 8 hours before performing muscle exhaustion exercises. At designated time points in the recovery period, including baseline (0) and 3h, 24h, and 48 after exercise, blood samples were collected with brachial venous catheters. Assess serum IL-6 levels (pg/ml) to monitor physiological fitness. Used commercial kit human IL-6 immunoassay (R&D system, Minnesota, USA) and Elisa reader (PerkinElmer, Massachusetts, USA) for analysis

  2. Clinical Biochemistry of CK level

    Time frame: 10 days

    To assess muscle damage, volunteers fasted for at least 8 hours before performing muscle exhaustion exercises. At designated time points in the recovery period, including baseline (0) and 3h, 24h, and 48 after exercise, blood samples were collected with brachial venous catheters. Serum CK (U/L), were assessed for monitoring physiological adaptation. All biochemical indices were assessed using an autoanalyzer (Hitachi 7060, Tokyo, Japan).

  3. Stress hormones 8-Hydroxy-2'-Deoxyguanosine (8-OHdG) in Urine

    Time frame: 10 days

    Subjects' urine was collected 24 hours after the exhaustive exercise for analysis. Used commercial kit human 8-OHdG (Cayman, Mich, USA) and Elisa reader (PerkinElmer, Massachusetts, USA) for analysis

  4. Assessment of muscle stiffness

    Time frame: 10 days

    3 h, 24h, 48h after exhausting exercise, use Myoton machine to evaluate muscle stiffness (N/m) (Myoton, Tallinn, Estonia)

  5. Visual Analogue Scale (VAS) evaluation

    Time frame: 10 days

    3 h, 24h, 48h after exhausting exercise, use VAS questionnaire to record muscle pain. the minimum is 0 and maximum is 10, and whether higher scores mean more pain

Sponsors and collaborators

Lead sponsor

National Taiwan Sport University

Other

Registry information

Official study title

Evaluation of the Efficacy of Supplementation With Lemon Verbena Extract in Improving

Important dates

Study start
2020
Primary completion
2020
Study completion
2020
First posted
Feb 8, 2021
Registry last updated
Mar 10, 2021

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

Related clinical trials

Published trials that share one or more normalized conditions with this study.