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OpenTrials
Completed

NCT Number: NCT04234295

Investigating the Effect of Obesity on Eosinophil Recruitment in Human Adipose Tissue

Researchers are trying to better understand the relationship between inflammation in adipose tissue (AT) and insulin resistance.

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Key information

Conditions

Obese Body Weight Healthy Nutrition Disorders Nutritional and Metabolic Diseases Obesity Overnutrition Overweight Pathological Conditions, Signs and Symptoms Signs and Symptoms

Age range

30 year–50 year

Sex eligibility

All sexes

Study type

Interventional

Phase

Not applicable

Primary location

Mayo Clinic

Scottsdale, Arizona, 85259, United States

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • Adults ages 30-50 years
  • Non-smokers
  • Lean subjects with a BMI of 25 or less, gender specific normal body fat,
  • Obese, non-diabetic subjects with a BMI between 30 and 50 and taking no medications affecting glucose metabolism or lipid metabolism
  • subjects scheduled for an elective laparoscopic or laparotomy abdominal surgery

Exclusion criteria

  • Receiving corticosteroid therapy or have a history of asthma, COPD or atopic syndrome
  • taking any biologic treatment for any autoimmune disorder
  • Taking any leukotriene receptor antagonist acting medications (ex. Montelukast)
  • smoking

Treatment and study plan

Fat Tissue Biopsy

Procedure

If subjects are scheduled for a surgical procedure a fat sample will be collected during the beginning of the surgery. If subjects are not scheduled for a surgical procedure, a small sample of subcutaneous (superficial) fat tissue from subjects abdomen area will be collected.

Primary outcomes

  1. Number of eosinophils in adipose tissue

    Time frame: baseline

    eosinophil number will be measured by flow cytometry in subcutaneous (superficial) adipose tissue

Secondary outcomes

  1. Correlation between eosinophil content and body mass index (BMI)

    Time frame: baseline

    Measured as a linear regression between BMI (defined as the weight in Kg/height in square m; kg/m2) and eosinophil number in the fat

Other outcomes

  1. Quantification of the difference in gene methylation in eosinophils from lean and obese subjects

    Time frame: baseline

    Gene methylation of eosinophils will be quantified in samples derived from lean and obese fat biopsy

Sponsors and collaborators

Lead sponsor

Mayo Clinic

Other

Registry information

Important dates

Study start
2020
Primary completion
2022
Study completion
2022
First posted
Jan 21, 2020
Registry last updated
Jan 19, 2022

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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