Completed

NCT Number: NCT04566354

Interleukin-6 Secretion in Adipose Tissue Following Sprint Exercise

Low volume sprint exercise training has been shown to reduce body fat despite small total energy expenditure (for ref see Gillen and Gibala 2014). IL-6 has been shown to increase adipose tissue lipolysis in vitro. Therefore, in an initial study on effects of sprint exercise on adipose tissue metabolism the investigators will examine acute short-term exercise effects on adipose tissue IL-6 exchange.

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Key information

Conditions

Metabolism in Adipose Tissue

Age range

20 year–40 year

Sex eligibility

All sexes

Study type

Interventional

Phase

Not applicable

Primary location

Karolinska Institutet/Karolinska University Hospital

Stockholm, 141 86, Sweden

About this study

PURPOSE: Low volume sprint exercise training has been shown to reduce body fat despite small total energy expenditure (for ref see Gillen and Gibala 2014). IL-6 has been shown to increase adipose tissue lipolysis in vitro. Therefore, in an initial study on effects of sprint exercise on adipose tissue metabolism the investigators examined acute short-term effects on adipose tissue IL-6 exchange.

METHODS: Four female and four male subjects perform repeated sprint exercise (3 x 30s with 20 min rest between; Wingate-test). Blood samples are repeatedly obtained, up to 120 min after the last sprint (9 min post ex), from catheters inserted percutaneously into brachial artery and a superficial subcutaneous vein on the anterior abdominal wall providing access to the venous drainage from the subcutaneous adipose tissue and analyzed for IL-6 by ELISA and lactate by spectrophotometry. Fat biopsies from the stomach will be obtained before, 15 min and 120 min after the last sprint. Blood flow will be determined by administration of Xenon-133 in the adipose tissue of the stomach. The release rate of Xenon-133 will be registrated continuously during the total investigation.

SUPPORT: This study is supported by grants from the Swedish National Center for Research in Sports

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

Physical active
Healthy

Exclusion criteria

Smokers
Pregnant
On medication
No infection
No asthma

Treatment and study plan

Repeated 30-s sprint exercise

Other

Analyze of Interleukin-6 concentration before and after repeated 30-s sprint exercise

Primary outcomes

Il-6 secretion in adipose tissue before and after 30-s repeated sprint exercise

Time frame: Before 30-s repeated sprint exercise

Stomach fat biopsies and arterial and stomach vein blood are obtained
Il-6 secretion in adipose tissue before and after 30-s repeated sprint exercise

Time frame: After 30-s repeated sprint exercise

Stomach fat biopsies and arterial and stomach vein blood are obtained

Secondary outcomes

Changes in blood flow in adipose tissue

Time frame: Before 30-s repeated sprint exercise

Xenon-133 is administered by a subcutaneous injection into the abdominal adipose tissue of approximately 1-2 MBq of Xenon-133 in the form of gas, at rest before work.
Changes in blood flow in adipose tissue

Time frame: After 30-s repeated sprint exercise

The rinsing rate of Xenon-133 from the tissue depot is continuously recorded during the study period by a non-invasive method using a portable scintillation detector system (Mediscint) up to 60 minutes after the last sprint work.
Changes in different metabolites

Time frame: Before 30-s repeated sprint exercise

Analysed for lactate, ammonia and IL-6
Changes in different metabolites

Time frame: After 30-s repeated sprint exercise

Analysed for lactate, ammonia and IL-6

Sponsors and collaborators

Lead sponsor

Karolinska Institutet

Other

Registry information

Official study title

Metabolism in Adipose Tissue Followed Following Sprint Exercise

Important dates

Study start: 2015
Primary completion: 2016
Study completion: 2017
First posted: Sep 28, 2020
Registry last updated: Sep 28, 2020

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

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This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.