Integrative Group Psychotherapy (Terebenin's Method) in Adults With Subclinical and Mild/Moderate Anxiety and Depression

Anxiety and depression are among the most prevalent mental health disorders worldwide, often presenting with overlapping symptoms that amplify the burdens on affected individuals and healthcare systems. According to the World Health Organization (WHO), depression alone affects over 280 million people globally (approximately 3.5% of the population). Anxiety disorders share a similar high prevalence and frequently co-occur with depressive conditions. In response to these challenges, diverse psychotherapy modalities have emerged, including integrative approaches that combine principles from different schools (e.g., psychodrama, Gestalt, family systemic, and body-oriented methods) to enhance treatment effectiveness. Meta-analyses indicate that these integrative strategies can achieve effect sizes (Cohen's d) in the range of 0.60-0.90, making them viable-or in some cases superior-alternatives to single-orientation therapies for mild to moderate depression ].

Within this integrative framework, Terebenin's Method stands out as a structured group intervention designed to address subclinical, mild, or moderate anxiety and depression. Its core elements include psychodrama-inspired enactments (allowing participants to "act out" internal conflicts or emotional challenges in a safe group context), systemic constellation techniques (focusing on relational and family-system dynamics through phenomenological exploration), body-oriented practices (emphasizing awareness of somatic tension and facilitating emotional expression), and a directive therapeutic style (where the therapist actively interprets and guides group processes) . The method posits that enacting and witnessing others' enactments of unconscious or maladaptive patterns facilitates emotional insight and symptom reduction, concurrently reinforcing self-esteem, resilience, and a sense of control.

Besides patient-reported outcomes, growing attention has turned to physiological markers that can provide objective evidence of treatment efficacy. Salivary cortisol (CORT) is widely recognized as an indicator of hypothalamic-pituitary-adrenal (HPA) axis function, known to rise under chronic stress, depressive episodes, and anxiety conditions. Likewise, salivary alpha-amylase (sAA) reflects sympathetic nervous system (SNS) activation and tends to increase during acute stress. Research suggests that effective psychotherapies can lower or normalize these biomarkers, thereby validating clinical improvements captured through self-report and observer-rated scales.

Against this background, the present study is designed as a pilot randomized controlled trial (1:1 allocation) to evaluate the effects of Terebenin's Method in individuals aged 18-60 who exhibit subclinical to moderate anxiety or depressive symptoms. The study will enroll approximately 60 participants (30 per group), enabling the collection of feasibility data and effect size estimates to inform a larger, fully powered RCT. Participants will be randomly assigned to one of two arms: (1) an 8-week course of integrative group therapy using Terebenin's Method (weekly sessions of 2-3 hours, led by 1-2 trained facilitators with a group size of 8-12), or (2) a control condition that may consist of an active psychotherapeutic intervention (e.g., short-term cognitive-behavioral therapy or Gestalt group) conducted on a similar 8-week schedule, or a waitlist if the active control is not feasible.

Inclusion criteria

require stable or no psychotropic medication use, absence of severe psychiatric disorders or acute suicidal risk, the ability to attend weekly sessions, compliance with saliva sampling, and written informed consent. Exclusion criteria encompass severe somatic illnesses preventing participation, current psychotherapy likely to confound results, and active substance use disorders unless in stable remission for more than six months.

Outcome measures will be collected at baseline (T0), post-intervention (~8 weeks, T1), and at follow-up (~1-3 months, T2). Primary clinical outcomes include depression scores (Beck Depression Inventory-II [BDI-II] or Hamilton Depression Rating Scale [HAM-D], administered by blinded evaluators) and anxiety scores (State-Trait Anxiety Inventory [STAI] or the anxiety subscale of the Hospital Anxiety and Depression Scale [HADS-A]). Secondary outcomes include quality of life (WHOQOL-BREF or SF-36), perceived stress (Perceived Stress Scale [PSS]), and therapeutic alliance (Working Alliance Inventory [WAI]). Physiological outcomes center on salivary cortisol and alpha-amylase, sampled three times a day (upon awakening, 30 minutes post-awakening, and in the evening) over two consecutive days at T0, T1, and T2. Key metrics will be the cortisol awakening response (CAR) and area under the curve (AUC) for both biomarkers.

Data management involves the use of standardized collection kits (e.g., Salivette®) and secure databases with unique participant IDs. Statistical analysis will follow an intent-to-treat approach, with repeated-measures ANOVA or mixed-effects models used to compare within- and between-group changes over time for both clinical and biomarker data. Normality violations may prompt nonparametric alternatives. Missing data will be handled via multiple imputation or last observation carried forward. A two-tailed alpha of p < 0.05 will be the threshold for statistical significance.

In summary, this pilot trial seeks to evaluate whether Terebenin's Method, an integrative group psychotherapy approach, yields clinically meaningful improvements in anxiety and depression while concurrently influencing objective biological markers of stress (salivary cortisol and alpha-amylase). The study's results will guide refinement of the intervention protocol, confirm or adjust effect size estimates, and lay the groundwork for a more extensive randomized controlled trial to rigorously test Terebenin's Method in larger and more diverse populations.

Inclusion criteria

• Adults aged 18-60.

• Subclinical, mild, or moderate anxiety/depressive symptoms (screened via HADS, BDI-II, STAI).
• No current psychotropic medication use or on a stable dose for ≥4 weeks with no planned changes.
• Ability to attend weekly group sessions for 8 weeks + follow-up visits, including saliva sample collection.
• Provision of written informed consent.

Exclusion criteria

• Severe psychiatric disorders (e.g., schizophrenia, bipolar disorder in a manic phase, major depression with psychotic features).

• Significant suicidal risk requiring urgent intervention.
• Severe somatic illness impeding participation.
• Active substance use disorder unless in stable remission for >6 months.
• Ongoing psychotherapy that could confound results.
• Inability to comply with saliva collection (e.g., shift work, severe sleep disturbances).