Integrated Clinical-molecular Risk Stratification to Early Recurrence in Colorectal Liver Metastases

Inclusion criteria

• Aged 18 to 75 years, inclusive (male or female).
• Pathologically and radiologically confirmed colorectal cancer with liver metastases.
• Must have undergone complete (R0) surgical resection of both the primary colorectal tumor and all liver metastases.
• Have adequate organ function and no contraindications to potential adjuvant therapies (surgery, chemotherapy, radiotherapy, immunotherapy).
• Willing and able to comply with the study protocol and follow-up visits.
• Availability of sufficient liver metastasis tumor tissue for gene sequencing.
• Provision of a blood sample for circulating tumor DNA (ctDNA) detection within one month after surgery but before starting any adjuvant therapy.

Exclusion criteria

• Pregnancy or breast-feeding women
• History of other malignancies within 5 years (except cured skin cancer and cervicalcancer in situ)
• History of uncontrolled epilepsy, central nervous system disease, or psychiatricdisorders
• Clinically serious heart disease, such as symptomatic coronary artery disease, NewYork Heart Association (NYHA) class II or worse congestive heart failure or severearrhythmia requiring pharmacologic intervention, or history of myocardial infarctionwithin the last 12 months
• Baseline blood and biochemical indicator do not meet the following criteria:neutrophils >=1.5×10^9/L, Hb >=90g/L, PLT >=100×10^9/L, ALT/AST<=2.5 ULN, Cr <= 1ULN
• Allergic to any component of the therapy
• Severe uncontrolled recurrent infections